A Study of the Tumour Cell Killing Effects in the Natural Killer and Lymphokine Activated Killer Cell Activity

1987 Fiscal Year Final Research Report Summary

• Project/Area Number: 61570961
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 外科・放射線系歯学
• Research Institution: Kanagawa Dental College
• Principal Investigator: JUNSHI Shindo Kanagawa Dental College, School of Dentistyr, Professor, 歯学部, 教授 (10084758)
• Co-Investigator(Kenkyū-buntansha): KAZUHIRO Kumegawa Kanagawa Dental College, School of Dentistry, Instuructor, 歯学部, 助手 (50170064) ; MIKIO Oohashi Kanagawa Dental College, School of Dentistry, Instructor, 歯学部, 助手 (00160599) ; YUUICHI Sasakura Kanagawa Dental College,School of Dentistry, Assistant Professor, 歯学部, 講師 (80121002) ; MISAO Yamaguchi Kanagawa Dental College, School of Dentistry, Assistant Professor, 歯学部, 講師 (80104438)
• Project Period (FY): 1986 – 1987
• Keywords: Mouse spleen cell / Mouse intra-liver cell / NK activity / LAK activity / IL-2 / DK-432

Research Abstract

Although we can not infuse to cancer patient with activated autolymphocytes, in this study we utilized mouse spleen cells and intra-liver cells in vitro to evaluate the function and the expressed cell membrane surface antigen of the IL-2 induced NK cell, LaK cell and OK-432 induced NK cell. The tumour cell killing activity was assayed by the YAC-1 cell and the P-815 cell which were goth target cells. These aforementioned P-815 cells used to evaluate tha LAK activity. The YAC-1 was used to assay the NK activ ity.
1 The IL-2 induced mouse spleen NK cell was evaluated on its meximum ability to kill the target cell after 4 days in culture with the aforementiones YAC-1 cell. The NK's killing activity was 95.6% in 50:1 E/T ratio and 94.6% in 20:1. The IL-2 induced mouse spleen LAK cell was evaluated on its maximum ability to kill the target cell after 4 days in culture with the aformewntioned P-815 cell. The LAK's killing activity was 48.6% in 50:1 E/T ratio and 42.3% in 20d1.
2 Thirty-six hours after IL-2 i.v. injecting labolatory mouse with OK-432, the mouse was destoyed and spleen was removed. The spleen cell were isolated and placed in a culture with the YAC-1 target cells. After 4 hours of culturing, the activity of these aformentioned spleen cell by OK-432 to kill the target cells was evaluated. The results of this killing activity were 16.5% in 25:1 E/T ratio, 27.4% in 50:1 and 41.8% in 100:1 We also assayed intra-liver NK cell killing activity in above same manner as the spleen cells. The intra-liver cell's killing activity resulted 55.9% in 25:1 E/T ratio, 63.1% in 50:1 and 65.2% in 100:1.
3. Note, by using anti-Thy1.2, anti L3T4, anti Lyt2, anti GA1, anti-KJ16, and anti-IL-2 receptor monoclonal antibodies, we can find out other intrersting facts about the two different IL-2 induced spleen cell surface antigens and the one of OK-432 induced intra-liver cell surface antigens.