1987 Fiscal Year Final Research Report Summary
The Effect of Some Serum Components on the Thiol-Dependent Collagenolytic Enzyme Isolated from Bacteroides gingivalis 381.
| Project/Area Number |
61570974
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ENDO Junko The University of Tokushima, Research Associate, 歯学部, 助手 (20176796)
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| Co-Investigator(Kenkyū-buntansha) |
HINODE Daisuke The university of Tokushima, Research Associate, 歯学部, 助手 (70189801)
SATO Makoto The university of Tokushima, Associate Proffessor, 歯学部, 助教授 (10126229)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Bacteroides gingivalis / Thiol-Dependent Collagenolytic Enzyme / 血清成分 |
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| Research Abstract |
Bacteroides gingivalis has been implicated in the etiology and pathogenesis of adult periodontitis. It is not doubtful that enzymes produced by the microorganism are of importance as the virulent factor. Our previous study demonstrated that culture media from B. gingivalis contained collagenolytic enzyme and that the enzyme activity was highly enhanced by the addition of reducing reagents having thiol groups. The purpose of this study is to elucidate the effect of some serum components on the collagenolytic enzyme from B. gingivalis.
Collagenolytic enzyme in culture media of B. gingivalis 381 was partially purified 115 fold with a specific activity of 467 Unit per mg protein by the procedures of aceton fractionation, gel filtration on Sepharose CL-6B followed by ion-exchange chromatography on DEAE-Sephacel. Human <gamma>-globulin, rabbit <gamma>-groubulin, human <alpha><_2>-macroglobulin, rabbit albumin, and anti B. gingivalis 381 rabbit <gamma>-globulin were used to examine the effects of some serum components on the partially purified collagenolytic enzyme from B. gingivalis. The collagenase activity was found to be strongly inhibited by not only globulin fractions but also by albumin. The phenomenon that some serum components strongly inhibited the collagenolytic activity, is of particular interest from the view point of oral defence mechanisms. However, it has been reported that some human serum components interact with some oral anaerobes including B. gingivalis and aggregate bacterial cells. In addition, several proteolytic enzymes, which is produced also by B. gingivalis, degrade serum components such as secretory Ig A and <alpha>_2-macroglobulin, and has no influence on the activity of proteolytic enzyme. As such, there might be several interactions among many factors in subgingival plaque and/or crevicular fluid. Therefore, further investigations are needed to elucidate the role of these serum components in periodontal disease.
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