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1989 Fiscal Year Final Research Report Summary

Rapid Diagnosis of Campylobacter Infection by Latex Agglutination Using Monoclonal Antibodies.

Research Project

Project/Area Number 61571118
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionShowa University

Principal Investigator

NAKAMURA Yoshiko  Showa University Fujigaoka Hospital, 医学部, 助教授 (70119238)

Co-Investigator(Kenkyū-buntansha) AOKI Yoshio  Showa University Fujigaoka Hospital, 医学部, 教授 (50053749)
Project Period (FY) 1986 – 1988
KeywordsCampylobacter jejuni / Monoclonal antibodies / Detection of antigens / Reversed passive latex agglutination / Western blotting / Detection of antibodies
Research Abstract

Campylobacter jejuni, the most frequent pathogens of acute gastroenteritis, usually requires two days for the isolation and identification. We made monoclonal antibodies(MoAb) against C. iejuni so as to search the frequencies of C. jejuni isolation and to establish the method for rapid diagnosis.
1) The frequencies of pathogens isolated from feces were 36% in C. jejuni/ coli, 27% in Klebsiella oxytoca, 14% in enteropathogenic E. coli and 9% in Salmonella spp.
2) Campylobacter coli shared 8% in all C. jejuni/coli.
3) Eleven anti C. jejuni monoclonal antibodies were obtained by means of heat extracted C. jejuni C_1 strain. The epitopes reactive with these MoAb were polypeptides having 45kDa(6 strains) and 65kDa(5 strains) molecular masses on SDS-PAGE. The classes of MoAb were identified as IgM(2 strains) and IgG(9 strains).
4) MoAb(H-194) sensitized latex agglutination(reversed passive latex agglutination, RPLA) was verified to be Campylobacter specific when examined by other pathogens of gastroenteritis.
5) The sensitivity of the RPLA was 4x10^4 CFU/ml, as calculated from the plate-RPLA and colony formation unit of C. jejuni C strain.
6) All clinically isolated 121 strains of C. jejuni and 11 strains of C. coli showed positive RPLA.
7) There was a 96%(249 of 260) total agreement between RPLA and the standard culture method. Among 200 feces showed positive culture, 198 were identified as positive RPLA. Nine feces among 60 negative culture showed positive results.
In summary, the RPLA appears to be useful for rapid detection(within 10 min) of Campylobacter. The method is sensitive, specific, simple to perform and requires no costly instrument.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 中村良子,他6名: "Campylobacter jejuni感染症の迅速診断法(第I報)ーmicro ELISAによる抗原検出法についてー" 臨床病理. 34. 277-281 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村良子,他6名: "Campylobacter jejuni感染症の迅速診断法(第II報)ーmicro ELISAによる血清抗体価測定ー" 臨床病理. 34. 454-458 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉田勝彦,他3名: "モノクロ-ナル抗体を用いたCampylobacter jejuni感染症の迅速検査" 衛生検査. 36. 649 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 丹羽朗,他3名: "Western Blot法によるCampylobacter jejuni感染症の血清診断" 臨床病理. 35. 99 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村良子,他6名: "カンピロバクタ-感染症の迅速診断(第III報)ーモノクロ-ナル抗体を用いた逆受身ラテックス凝集反応による抗原検出法についてー" 臨床微生物迅速診断研究会誌. 1. 47-56 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshiko Nakamura, Setsuko Tazawa, Takeo Sawada, Isao Yonekubo, Ichiro Fukuda, Kazuo Okumura and Yoshio Aoki: "Rapid detection of Campylobacter jejuni by enzyme linked immunosorbent assay in the patients with acute enteritis(I)." the Japanese Journal of Clinical Pathology. 34. 277-281 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiko Nakamura, Takeo Sawada, Isao Yonekubo, Setsuko Tazawa, Atsushi Takeda, Yoshio Aoki and Hazime Sato: "Rapid detection of anti-Campylobacter jejuni antibodies by enzyme linked immunosorbent assay(II)." the Japanese Journal of Clinical Pathology. 34. 454-458 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiko Nakamura, Atsushi Takeda, Atsuro Iwasawa, Yoshio Aoki, Katsuhiko Yoshida, Akira Niwa and Setsuko Tazawa: "Rapid diagnosis of Campylobacter infection by latex agglutination using monoclonal antibodies." Journal of the Association for Rapid and Automation in Microbiology. 1. 47-56 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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