1987 Fiscal Year Final Research Report Summary
X-Ray Crystallographic Analysis of Spherical Plant Viruses
| Project/Area Number |
61580049
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
結晶学
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| Research Institution | Faculty of Science, Osaka University (1987)
Tottori University (1986) |
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Principal Investigator |
FUKUYAMA Keiichi Faculty of Science, Osaka University, 理学部, 講師 (80032283)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKIHARA Tomitake Faculty of Engineering, Tottori University, 工学部, 助教授 (00032277)
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| Project Period (FY) |
1986 – 1987
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| Keywords | Spherical Virus / Tobacco Necrosis Virus / X-Ray Analysis / 結晶構造 |
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| Research Abstract |
Tobacco necrosis virus (TNV) is a spherical plant virus consisting of 180 copies of coat protein and a single-stranded RNA. TNV was propagated in Chenopodium quinoa, and purified by differential centrifugation, gel filtration and sucrose density centrifugation. The purified TNV showed only one band at M=30,000 in SDS-polyacrylamide gel electrophoresis, and contained homogeneous particles of a diameter of approximately 28 nm in the electron microscope. TNV crystals were produced by dialyzing against 0.4 M sodium phosphate buffer with the pH adjusted to 6.0. The TNV crystals grew in the form of dodecahedron, to a maximum of 1 mm in diameter. The diffraction patterns of the crystals have shown that the crystal belongs to cubic space group p4_232 with a=338 <Ang>. The two particles in the unit cell are rotated 90 to each other about the principal axis of the crystal situated at (0,0,0) and (1/2,1/2,1/2). The nearest distance between the particles in the unit cell is 293 <Ang>. The crystals diffract X-ray to 2.5 <Ang> resolution and showed little radiation damage. Collection of three-dimensional X-ray data and preparation of heavy atom derivatives are in progress.
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