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1987 Fiscal Year Final Research Report Summary

Molecular chractrerization of cell aggregation factor(s) in celluar fibronectin prepatration - A possibility of a determinant chondrogene

Research Project

Project/Area Number 61580136
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionAichi Medical University (1987)
Nagoya University (1986)

Principal Investigator

KIMATA Koji  Aichi Medical University, IMSM. Assistant Professor, 分子医科学研究所, 助教授 (10022641)

Project Period (FY) 1986 – 1987
KeywordsChondrogrnesis / Mesenchymal cell condensation / Fibronectin / beta-galectosidebinding lectin / Hemagglutination / Tenashin / 細胞外マトリックス
Research Abstract

Cellular fibronection prepared from chick embryonic fibroblast cells (CCFN) has been shown to have the active suvstance(s) to promote in vitro aggregation of chick embryonic limb bud mesencymal cells and subsequent chondrogrmesis of the aggregated cells. When fractionated by Sepharose CL 2b column chromatography, we found the activity in the lower molecular weight fraction of CCFN which gave a few glycoprotein bands around Mr=15,000 on SDS-polyacrylamide gel electrohoresis. Kasai et al(Teikyo University) have demonstrated the existence of tw kinds of beta-galactoside-binding lectins having Mr=16,000 and Mr=14,000, respectively, in check embryo. These lectins and their specific antibodies were served to see whether the active substance(s)in CCFN is identical with these lectine ot not. SDS-polyacrylamide gel electrophoresis and subsequent immunoblotting using these antibodies suggested the presence of not only both the Mr=16,000 and Mr=14,000 lectins in CCFN but also the Mr=16,000 lCtin in the detergent extract of check embryo limb buds at stages 22-23. The difect addition of the putified lectine to the culture medium of the limb bud mesenchymal cells prometed the cell aggregation. The simultaneous addition of either the antibodies with CCFN neutralized the induced cell aggregation. Furthermore, the addition of the antibodies to the culture without CCFN brought about the complete disappearance of the slowly going cell aggregation which was probably due to the endogenous synthesis of the lection. Therefore, it is likely that the active substrances in CCFN is caused by the contaminants, Mr=16,000 and 14,000 beta-galactoside-binding lectins and that the former lectin exists in the limb buds and plays an importanr role in the cell aggregation during limb bud mesenchymal condensation.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Koji Kimata: J.Biol.Chem.261. 13017-13525 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Yamagata: J.Biol.Chem.261. 13526-13535 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satomi Ono: Development,Growth and Differentiation.28. 401-401 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junitiro Funahashi: Development,Growth and Differentiation.30. (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satomi Ono: (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koji Kimata: (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木全弘治: "器官形成研究会編, 器官形成(発生生物学から臓器工学まで)第3章プロテオグリカンと接着性糖タンパク質" (培風館), 15 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimata, K., Oike Y., Tani, K.,Shinomura, T., Yamagata, M., Uritani, M., and Suzuki, S.: "A large chondroitin sulfate proteoglycan (PG-M) synthesized bedore chondrogenesis in the limb bud of check embryo" J. Boil. Chem.261. 13517-13525 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamagata, M., Yamada K. M., Yoneda, M., Suzuki, S., snd kimata,K: "Chondroitin sublfate proteglycan (PG-M-like proteoglycan) in involved in the binding of hyalurnic acid to celluar fibronectin" J. Biol. Chem.261. 13526-13535 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ono. S., Kimata, K., Shimomura, Y., and Suzuki, S.: "Mesenchymal cell condensation during cartilage differentiation Role og the extracellular matrix molecules" Development, Grouwht p Differentiation. 28. 401-401 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Funahashi, J., Asano, Y., Suzuki, S., Kimata, K., hitabayashi, J., Oda, Y., and Kasai, K.,: "Chondrogrnensis and mesencymal cell condensation - Participation of lectin-like molecules" Development, Growth & Differetiation. In press (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ono, S., Karasawa, K., and Kimata,K: "A cellualr fibronection prepatation promotes chondrogenesis of check limb bud mesenchymal cells in vitro"

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimata K: Baifukan. Proteoglycans and ashesive glycoproteins in "Organ formation", 15 (1987)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1989-03-30  

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