1988 Fiscal Year Final Research Report Summary
Decalcification and Immunohistochemical Innovation for Calcified Tissues
| Project/Area Number |
61870020
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
KYOGOKU M. Tohoku University School of Medicine, 医学部, 教授 (70025542)
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| Co-Investigator(Kenkyū-buntansha) |
TESHIMA T. Tohoku University School of Densitry, 歯学部, 教授 (50005089)
SAWAI T. Tohoku University School of Medicine, 医学部, 講師 (00125577)
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| Project Period (FY) |
1986 – 1988
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| Keywords | Decalcification / Immunoperoxidase method / Cell surface antigen / Monoclonal antibody / Immunoelectron microsocpy / Human |
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| Research Abstract |
We have developmed a new decalcifying technique for use in light and electron microscopy studies utilizing immunohistochemical staining of calcified tissues. Specimens containing calcified tissues can be adequately decalcified at a pH of 7.1-7.4 and temperature of -5゜C, without freezing, by use of a solution containing EDTA, sodium hydroxide, and glycerol. In this study, Leu-2a, Leu-3a, Leu-4, Leu-7, Leu-12, Leu-14, Leu-M1, Leu-M2, Leu-M3, and HLA-DR-positive cells in destructive lesions of bone tissues from patients with rheumatoid arthritis and ostemyelitis were successfully detected immuno-histochemically. Furthermore, the presence of HLA-DR antigen on the surface of the infiltrating cells in the same lesions could be demonstrated using the immunoelectron microscopic technique. The results reported here have not previously been obtainable using conventional decalcifying techniques.
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