1988 Fiscal Year Final Research Report Summary
Development of quantitative measurement instrument of protein concentration and number of cells in the aqueous by laser
| Project/Area Number |
61870070
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | University of Tokyo (1988)
Jichi Medical University (1986-1987) |
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Principal Investigator |
SAWA Mitsuru Section of Corneal Transplantation, University Hospital University of Tokyo School of Medicine, 医学部(病), 助教授 (40010475)
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| Co-Investigator(Kenkyū-buntansha) |
市橋 直 興和株式会社, 調布研究所, 研究員
柿沢 弘一郎 興和株式会社, 調布研究所, 研究課長
ITO Eiichi Research Institute of Kowa Co., 調布研究所, 所長
OKUBO Akira Department of Ophthalmology, Jichi Medical School, 医学部, 助手 (70125756)
TSURU Tadahiko Department of Ophthalmology, Jichi Medical School, 医学部, 講師 (90126128)
KAKIZAWA Koichiro Research Institute of Kowa Co.
ICHIHASHI Tadashi Research Institute of Kowa Co.
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| Project Period (FY) |
1986 – 1988
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| Keywords | Quantitative measurement / aquoues / protein concentration / cell count / in vivo / He-Ne laser / photon counting photomultiplier / computer / beam scan |
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| Research Abstract |
We have developed a new quantitative method to determine protein concentration and number of cells in the aqueous in vivo. The principal instruments in the system were a He-Ne laser and detection system for measuring scttered light intensity. The power of the He-Ne laser was 25muw and the focused beam diameter was 20mum. The laser beam was operated by an optical scanner. The sampling window, 0.3x0.5 mm, was fixed in the center of the laser path. For the protein concentration measurement, the laser beam was scanned for a length of 0.6mm vertically, covering the sampling window. For the cell counts, the laser beam(0.25 x 0.6mm) was scanned over the sampling window and the detected peaks were counted. Each measurement mode took 0,5 seconds. The operation of the instrument and data analysis were performed by a personal computer. In in vitro measurements with bovine albumin solution in concentrations ranging from 1g/100 ml to 1mg/100 ml, significant linear correlations between the values for concentration and photon counts(/msec) were obtained. In in vitro experiments with latex particles with diameter of 2.02 or 2.95um, significant correlations between the number of latex particles and number of detected peaks were also obtained. Clinical application of the instrument could reveal pathophyiology of not only postoperative inflammation but also endogenous uveitis. It could also investigate the effects of anti-inflam-matory drugs on these inflammation in the anterior segment of the eye.
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