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1988 Fiscal Year Final Research Report Summary

Development of chromosome sorting techniques using a dual laser chromosome sorter

Research Project

Project/Area Number 61880023
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionOsaka University

Principal Investigator

MATSUBARA Kenichi  Osaka University,Professor, 細胞工学センター, 教授 (20037394)

Co-Investigator(Kenkyū-buntansha) OCHIYA Takahiro  Osaka University,Research Associate, 細胞工学センター, 助手 (60192530)
FUKUSHIGE Shinichi  Osaka University,Research Associate, 細胞工学センター, 助手 (90192723)
Project Period (FY) 1986 – 1988
KeywordsCell Sorter / Dual Laser / 染色体
Research Abstract

We have developed chromosome sorting techniques using a dual laser chromosome sorter to analyze the structure of human chromosomes.
Human chromosomes stained with Hoechst 33258 (Ho) and chromomycin A3 (CA3) flowed sequentially through two laser beams, one adjusted to 457.9nm to excite CA3 and the other adjusted to 351.1-363.8nm to excite Ho. The Ho and CA3 contents of individual chromosomes were determined by measuring the intensities of fluorescence that were emitted as they passed two laser beams. These measurements were accumulated to form fluorescence intensity distributions.
Sorting techniques have evolved from the ability to sort 10-20 human chromosome fractions with a single laser system to sorting 21 fractions including 15 unique types with a dual laser system.
dFurthermore, chromosome types that cannot be sorted at high purity from chromosomes isolated from human cells can be sorted from human-hamster hybrid cell lines containing one or a few human chromosomes.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Fukushige,Shinichi: Biochem.Biophys.Res.Commun.134. 477-483 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukushige,Shinichi: Mol. Cell.Biol.6. 955-958 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokino,Takashi: J.Virol.61. 3848-3854 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horii,Akira: Biochem.Biophys.Res.Commun.149. 635-641 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 福重真一: 細胞工学. 7. 896-903 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村紀彦: 大阪大学医学雑誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukushige,Shinichi: "Chromosomal assignment of human genes for gastrin, thyrotropin (TSH)- subunit and c-erbB-2 by chromosome sorting combined with velocity sedimentation and Southern hybridization" Biochem.Biophys.Res.Commun.134. 477-483 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukushige,Shinichi: "Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line" Mol.Cell.Biol.6. 955-958 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokino,Takashi: "Chromosomal translocation and inverted duplication associated with integrated hepatitis B virus in hepatocellular carcinomas" J.Virol.61. 3848-3854 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horii,Akira: "Primary structure of human pancreatic secretory trypsin inhibitor (PSTI) gene" Biochem.Biophys.Res.Commun.149. 635-641 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura,Norihiko: "Identification of human chromosomes using human endogenous retroviral LTRs as marker probes" Medical Journal of Osaka University Japanese Edition,.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-12-19  

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