1988 Fiscal Year Annual Research Report

筋肉に於けるATP分解から収縮に至る過程の研究

Research Project

Project/Area Number	62304032
Research Institution	Medical College of Oita
Principal Investigator	山田和廣大分医科大学, 医学部, 教授 (20053027)
Co-Investigator(Kenkyū-buntansha)	柳田敏雄大阪大学, 基礎工学部, 教授 (30089883) 小川靖男順天堂大学, 医学部, 教授 (50103841) 富田忠雄名古屋大学, 医学部, 教授 (50078763) 杉晴夫帝京大学, 医学部, 教授 (20082076) 江橋節郎国立岡崎生理学研究所, 所長 (10009863)
Keywords	アクチンフイラメント / ATP分解 / スキンドファイバー / caged ATP / caged Ca / 燐NMR法
Research Abstract	1.ミオシン頭部および筋原線維におけるATP分解と収縮。柳田は、ミオシン頭部を付着させたガラス板上を、アクチンフイラメントの滑走する速さに、ATP分解との関係を調べ、筋収縮の分子機構の本質に関する重要な発見を行った。この結果はこれまでのどのような仮説とも相容れないことから、筋収縮の分子機構に関する新しい仮説の提唱に至った。馬詰、児玉及び太和田は上の結果の検証のために筋原線維のレベルで研究を行った。小川は薬物を介入させることによる、収縮とATP分解の関係の修飾についての研究に成功した。 2.細胞膜剥離標本(スキンドファイバー)及びグリセリン処理筋におけるATP分解と収縮。飯野はスキンドファイバーの収縮に対する無機燐酸の効果について、従来の仮説とは相容れない複雑な機構の関与を示した。松原は高速X線回折法により、心筋と骨格筋の違いについての知見を得た。山田、米谷及び杉は、caged ATP及びcaged Caを用いる研究を開始することができた。 3.筋線維におけるATP分解と収縮。山田及び杉は骨格筋について燐NMR法により収縮とATP分解の関係を調べ、山田はATPの収縮分解現象を確立した。また、杉、は解糖系の賦活について一歩進めた知見を得た。 4.平滑筋における収縮の賦活の特殊性。江橋及び藪は、平滑筋における収縮の制御の特殊性を調べ、重要な知見を加えた。 5.筋病学との関連性。杉田は、マッカードル病における筋障害と、筋細胞内のアルカリ化との関係を調べ、病因及び発症機序についての重要な発見を行った。この他ジストロフィー症の原因についても重要な発見を行った。

Research Products

(27 results)

All Other

All Publications (27 results)

[Publications] Kawano,et al.: J.Physiol.(London). 407. 243-261 (1988)
[Publications] Yamada,K,;Tanokura,M.: Proc.Acad.Japan. 64. 278-281 (1988)
[Publications] Ebashi,S,;Ogawa,Y.: Biophysical Chemistry. 29. 137-143 (1988)
[Publications] Horiuti,K,et al.: J.Muscle.Res.cell Motil.9. 156-164 (1988)
[Publications] Matsumura,M.: Abstract of the III International Conference on Muscle Energetics.
[Publications] Tomita,T.: Jpn.J.Physiol.38. 1-18 (1988)
[Publications] Nakayama,S.,et al.: J.Physiol.(London). 402. 565-578 (1988)
[Publications] Yoshino,M.,et al.: Plugers Archiv.411. 229-231 (1988)
[Publications] Takagi,A.,et al.: Biochemical Res.9. 243-246 (1988)
[Publications] Araki,M.,et al.: Neurology. 38. 297-301 (1988)
[Publications] Emoto,Y.;Tawada,K.: J.Biochem.103. 172-176 (1988)
[Publications] Tsukioka,M.,et al.: Jpn.J.Physiol.46. 311 (1988)
[Publications] 児玉孝雄: 熱測定. 115. 2-9 (1988)
[Publications] Maruyama,T.,et al.: J.Biochem.(1989)
[Publications] 柳田敏雄: 科学. 58. 477-485 (1988)
[Publications] Koshino,A.;Yanagida,T.: Nature. 334. 74-76 (1989)
[Publications] Kurebayashi,N.: J.Physiol.(London). 403. 407-424 (1988)
[Publications] Ebashi,S.;Ogawa,Y.: "Toroponin C and calmodulin as calcium receptors; mode of action and sensitivity to drugs.in Handbook of Experimental Phamacology" Springer -Verlay, 31-56 (1988)
[Publications] Yamada,T.;Sugi,H.: "^<31>P NMR study of the regulation of glycogenolysis in iodoacetate-treated frog skeletal muscle.in Molecular Mechanism of Muscle Contraction" Plenum, 449-456 (1988)
[Publications] Yamada,T.;Sugi,H.: "Regulation of gylcogenolysis in contracting frog skeletal muscle studied by ^<31>P nuclear magentic resonance.in Muscle Energetics" Alan R.Liss, (1989)
[Publications] Matsubara,I.,et al.: "Cross-bridge movement in murine cardiac muscle as a function of calcium concentration.in Muscle Energetics" Alan R.Liss, (1989)
[Publications] Higuchi,H.,et al.: "Suppressing effect of 2,3-butanedione monoxime on contraction and ATPase activity of rabbit skeletal muscle.in Muslce Energetisc" Alan R.Liss,
[Publications] Yoshino,M.,et al: "Voltage-dependent Ca channels in smooth muscle membrane.in CALCIUM SIGNAL AND CELL RESPONSE" Springer-Verlag, 214-216 (1988)
[Publications] Tawada,K.;Emoto,Y.: "Rigor tenstion development in gylcerinated rabbit psoas fibers of high salt concentrations.in Molecular Mechanisms of Muscle Contraction(Sugi,H.＆ Pollak,G.H.,eds)" Plenum, 1988
[Publications] Endo,et al.: "Effects of inorganic phsophate on tension production in skinned skeletal muscle fibers.in Muscle Energetics(Pawl,R.,Elainga,G.＆ Yamada,K.,eds)" Alan R.Liss,
[Publications] Maruyama,et al.: "Effects of ethyler.qlycol on contractile properties of glycerinated rabbit pa as muscle.in Muscle Energetics(Paul,R.,Elainga,G.＆ Yamada,K.,eds)" Alan R.Liss,
[Publications] Ogawa,Y.;Kurebayashi,N.: "Modulations by drugs of the relationship between calcium binding to troponin C and tension.in Muscle Energetics." Alan R.Liss,

1988 Fiscal Year Annual Research Report

筋肉に於けるATP分解から収縮に至る過程の研究

Principal Investigator

山田 和廣 大分医科大学, 医学部, 教授 (20053027)

Research Products

[Publications] Kawano,et al.: J.Physiol.(London). 407. 243-261 (1988)

[Publications] Yamada,K,;Tanokura,M.: Proc.Acad.Japan. 64. 278-281 (1988)

[Publications] Ebashi,S,;Ogawa,Y.: Biophysical Chemistry. 29. 137-143 (1988)

[Publications] Horiuti,K,et al.: J.Muscle.Res.cell Motil.9. 156-164 (1988)

[Publications] Matsumura,M.: Abstract of the III International Conference on Muscle Energetics.

[Publications] Tomita,T.: Jpn.J.Physiol.38. 1-18 (1988)

[Publications] Nakayama,S.,et al.: J.Physiol.(London). 402. 565-578 (1988)

[Publications] Yoshino,M.,et al.: Plugers Archiv.411. 229-231 (1988)

[Publications] Takagi,A.,et al.: Biochemical Res.9. 243-246 (1988)

[Publications] Araki,M.,et al.: Neurology. 38. 297-301 (1988)

[Publications] Emoto,Y.;Tawada,K.: J.Biochem.103. 172-176 (1988)

[Publications] Tsukioka,M.,et al.: Jpn.J.Physiol.46. 311 (1988)

[Publications] 児玉孝雄: 熱測定. 115. 2-9 (1988)

[Publications] Maruyama,T.,et al.: J.Biochem.(1989)

[Publications] 柳田敏雄: 科学. 58. 477-485 (1988)

[Publications] Koshino,A.;Yanagida,T.: Nature. 334. 74-76 (1989)

[Publications] Kurebayashi,N.: J.Physiol.(London). 403. 407-424 (1988)

[Publications] Ebashi,S.;Ogawa,Y.: "Toroponin C and calmodulin as calcium receptors; mode of action and sensitivity to drugs.in Handbook of Experimental Phamacology" Springer -Verlay, 31-56 (1988)

[Publications] Yamada,T.;Sugi,H.: "^<31>P NMR study of the regulation of glycogenolysis in iodoacetate-treated frog skeletal muscle.in Molecular Mechanism of Muscle Contraction" Plenum, 449-456 (1988)

[Publications] Yamada,T.;Sugi,H.: "Regulation of gylcogenolysis in contracting frog skeletal muscle studied by ^<31>P nuclear magentic resonance.in Muscle Energetics" Alan R.Liss, (1989)

[Publications] Matsubara,I.,et al.: "Cross-bridge movement in murine cardiac muscle as a function of calcium concentration.in Muscle Energetics" Alan R.Liss, (1989)

[Publications] Higuchi,H.,et al.: "Suppressing effect of 2,3-butanedione monoxime on contraction and ATPase activity of rabbit skeletal muscle.in Muslce Energetisc" Alan R.Liss,

[Publications] Yoshino,M.,et al: "Voltage-dependent Ca channels in smooth muscle membrane.in CALCIUM SIGNAL AND CELL RESPONSE" Springer-Verlag, 214-216 (1988)

[Publications] Tawada,K.;Emoto,Y.: "Rigor tenstion development in gylcerinated rabbit psoas fibers of high salt concentrations.in Molecular Mechanisms of Muscle Contraction(Sugi,H.＆ Pollak,G.H.,eds)" Plenum, 1988

[Publications] Endo,et al.: "Effects of inorganic phsophate on tension production in skinned skeletal muscle fibers.in Muscle Energetics(Pawl,R.,Elainga,G.＆ Yamada,K.,eds)" Alan R.Liss,

[Publications] Maruyama,et al.: "Effects of ethyler.qlycol on contractile properties of glycerinated rabbit pa as muscle.in Muscle Energetics(Paul,R.,Elainga,G.＆ Yamada,K.,eds)" Alan R.Liss,

[Publications] Ogawa,Y.;Kurebayashi,N.: "Modulations by drugs of the relationship between calcium binding to troponin C and tension.in Muscle Energetics." Alan R.Liss,

山田和廣大分医科大学, 医学部, 教授 (20053027)