1988 Fiscal Year Final Research Report Summary
Analyses of drug respnse and signal transduction in the membrance of mediator producing cells
| Project/Area Number |
62304052
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | University of Tokyo |
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Principal Investigator |
NATORI Shunji Faculty of Pharmaceutical Sciences, University of Tokyo, Professor, 薬学部, 教授 (50012662)
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| Co-Investigator(Kenkyū-buntansha) |
FUTAI Masamitsu Institute of Industrial Science, Osaka University Professor, 産業科学研究所, 教授 (50012646)
SADAHIKO Ishibashi Faculty of Medicine, Hiroshima University, Professor, 医学部総合薬学, 教授 (90012616)
ICHIKAWA Atsushi Faculty of Pharmaceutical Sciences, Kyoto University, Professor, 薬学部, 教授 (10025695)
AKAMATSU Yuzuru The National Institute of Health, Department of Chemistry, Director, 化学部, 部長 (00072900)
INOUE Keizo Faculty of Pharmaceutica Sciences, University of Tokyo, Professor, 薬学部, 教授 (30072937)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Mediator / Sarcophaga lectin / PAF / prostaglandin / superoxide / LPS / Lipid A / ATPase / mast Cells / 胃酸分泌細胞 / リセプター |
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| Research Abstract |
This project was designed to analyze receptors of various bioactive molecules. Natori studied Sarcophaga lectin receptor on the sufface of mouse macrophages, and he identified two proteins (subunit) with molecular masses of 170 kDa and 110 kDa. Akamatsu investigated the mode of action of lipid a on murine macrophages. He synthesized various lipid A analogues and examined structure-acitivity relationship of lipid A. Inoue studied the mechanism of platelet activation by PAF, and found that phospholipase A_2 plays a cyucial role in the activation of platelet. Ichikawa analyzed pvostaglandin binding site on the surface of mast cells, and found that it tightly attach to membrane g-protein. Ishibashi found a new 46 kDa protein in the membrane of polymorphonuclear leukocytes. This protein was shown to be phosporylated when superoxide was produced by these cells, in response to various stimuli. Futai purified a H^+ /K^+ ATPase from gastvic mucosa of pig and determined complete amino acid sequence by isolating cDNA for this protein. He also pointed out that essential amin acid residues responsible for the ATPase activity.
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