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1989 Fiscal Year Final Research Report Summary

Environmental Control in Biological Engineering

Research Project

Project/Area Number 62440018
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 農業気象・生物環境制御学
Research InstitutionThe University of Tokyo

Principal Investigator

TAKAKURA Tadashi  Univ. of Tokyo, Dept. of Ag. Eng., Professor, 農学部, 教授 (50011929)

Co-Investigator(Kenkyū-buntansha) MORISHIMA Hiroshi  Univ. of Tokyo, Dept. of Ag. Eng., Professor, 農学部, 教授 (90011832)
GOTO Eiji  Univ. of Tokyo, Dept. of Ag. Eng., Instructor, 農学部, 助手 (00186884)
HONJO Tsuyoshi  Univ. of Tokyo, Dept. of Ag. Eng., Instructor, 農学部, 助手 (60173655)
CHINO Mitsuo  Univ. of Tokyo, Dept. of Ag. Chm., Professor, 農学部, 教授 (10007677)
KURATA Kenji  Univ. of Tokyo, Dept. of Ag. Eng., Assoc. Prof., 農学部, 助教授 (90161736)
Project Period (FY) 1987 – 1989
KeywordsBiotechnology / Masspropagation / Tissue culture / Enbryogenic cells
Research Abstract

Automatic screening system for enbryogenic cells and tissue culture vessels by means of ultrasonic misting have been developed in the present research year.
Through the stage of callus, carrot somatic embryos were developed and they were screened through two kinds of meshes. the flow of liquid media was switched in different ways in order to prevent blocking of meshes.
One experiment consisted of four continuous trials. Although not many cells can be obtained in the first two experiments, in the third trial the ratio of cells in the torpedo stage to the total cells over 300 micron meter was 163/683. It is concluded that the ratio of normal cells is relatively high and the blocking of meshes is not serious.
Cymbidium PLB and potatoes were used for misting culture. It was found that there is no difference between the fresh weight of Cymbidium PLB in misting culture and that of liquid culture and absorption of sugar and water can be controlled by misting system although the dry weight in misting system is inferior.
In potato cultivation, all vegetable growth in misting system is superior to the other.
It would be necessary to solve high humidity condition in vessels as well as lower light intensity caused by water droplets on the vessel surfaces although the absorption of water and sugar is easily controlled by misting intervals.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 本條毅: "植物組織培養によるシンビジゥムPLB増殖へのCO_2濃度光強度および液体培地組成の影響" 農業気象. 43. 223-227 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 後藤英司: "葉菜類の人工光栽培における光強度と照射コストの関係" 農業気象. 43. 229-232 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honjo,T.: "Effect of CO_2 enrichment on the growth of CYMBIDIUM PLB in vitro" Acta Horticulturae. 230. 185-188 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 孫禎翼: "植物工場におけるサラダ菜の蒸散量とチップバ-ンに対する培養液の電気伝導度と光条件の影響" 農業気象. 44. 253-258 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 孫禎翼: "ジャ-ファ-メンタ培養におけるCO_2施用が培地のpHに及ぼす影響" 農業気象. 45. 1-5 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 蔵田憲次: "超音波ミストを利用した組織培養装置の試作" 日本農業気象学会講演要旨. 18-19 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honjo, T., and Takakura, T.: "Effects of CO_2 concentration, light intensity and liquid medium composition for the growth of Cymbidium PLB in vitro." J. Agr. Met.43. 223-227 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goto, E., and Takakura, T.: "Relationship between light intensity and light energy requirement in the growth of leaf vegetables under artificial light." J. Agr. Met., 43, 229-232 1987.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Son, J.E., Kim, M.K., Honjo, T., and Takakura, T.: "Influence of CO_2 enrichment in jar fermentor culture on the pH of culture media" J. Agr. Met., 45, 1-5, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Son, J.E., and Takakura, T.: "Effect of EC of nutrient solution and light condition on transpiration and tipburn injury of lettuce in a plant factory." J. Agr. Met., 44, 253-258 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honjo, T., and Takakura, T.: "Effect of CO_2 enrichment on the growth of CYMBIDIUM PLB in vitro." Acta Hort., 230, 185-188 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurata, K., Jyuni, T., Ibaraki, Y., and Goto, E.: "Development of tissue culture vessels by means of ultrasonic misting." Annual Meeting of Agr. Met. 18-19, 1989.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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