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1990 Fiscal Year Final Research Report Summary

Mechamisms of PAF-Induced Airway Hyperresponsiveness in Bronchial Asthma

Research Project

Project/Area Number 62440038
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionDokkyo University School of Medicine

Principal Investigator

MAKINO Sohei  Dokkyo University School of Medicine Professor, 医学部, 教授 (10049140)

Co-Investigator(Kenkyū-buntansha) ARIMA Masahumi  DUSM Fellow, 医学部, 助手 (00202763)
YUKAWA Tatsuo  DUSM Lecturer, 医学部, 講師 (80158336)
MOTOJIMA Shinji  DUSM Lecturer, 医学部, 講師 (90157842)
FUKUDA Takeshi  DUSM Associate Professor, 医学部, 助教授 (90088873)
Project Period (FY) 1987 – 1990
KeywordsPAF / Eosinophil / Airway hyperresponsiveness / Bronchial asthma / Mouse / Guinea pig / PAF antagonist / Cellular immunity
Research Abstract

Research Results : (1) Effects of selective RAF antagonist, WEB2086 on late phase airway narrowing and post-latephase airway hyperresponsiveness (AH) : We already found that antigen exposure caused immediate and latephase AN and post latephase AH in actively sensitized guinea pig. In order to know the roles of PAF on these airway responses, we pretreated actively sensitized guinea pigs and challenged the animals with a specific antigen of ovalbumine (OA). Both latephase AN and post-latephase AH were inhibited significantly, suggesting that PAF might play significant roles in-AH observed in atopic asthma patients. (2) Enhanced PAF-induced eosinophilia in actively sensitized mice, but not in not-sensitized animals. In actively sensitized guinea pigs the inhalation of a specific antigen causes eosinophil infiltration in the bronchial mucosa which last as long as 5 days, while such prolonged eosinophilia is not observed in passively sensitized guinea pig. Since one of the difference of actively sensitized guinea pigs as compared to passively sensitized animals, is the presence of sensitized T-lymphocytes which may activate and prolong the survival of eosinophils. In order to study the role of active sensitization on PAF-induced eosinophil chemotaxis, BALB/C mice were sensitized by intraperitra injection of OA and challenged with intradermal PAF (long/site). Eosinophil infiltration in the skin was measured 6 hours after the intradermal PAF injection 0 and 15 as found that the infiltration was higher in actively sensitized mice as compared to not-sensitized normal mice. These observations suggest that PAF-induced eosinophil infiltration was enhanced possible T-cell derives cytokine, including IL-5. These observation shows possible clinical appreciation of PAF antagonist and suppressing agents in T-cell functions in the treatment of chronic asthma.

  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 福田 健,阿久津 郁夫,天下井 正弘,沼尾 利郎,本島 新司,牧野 荘平: "モルモット喘息モデルでの2相性好酸球浸潤に対する PAF拮抗剤とCyclosporin Aの効果" アレルギ-. 39(6). 548-552 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 阿久津 郁夫,福田 健,沼尾 利郎,牧野 荘平: "モルモット実験喘息モデルにおける遅発型喘息反応の出現と気道反応性亢進のFKー506による抑制" アレルギ-. 39(7). 605-609 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 有馬 雅史,湯川 龍雄,寺師 義典,相良 博典,牧野 荘平: "モルモット実験喘息モデルにおける遅発型反応と気道過敏性亢進に及ぼすWEB2086の影響" アレルギ-. 40(2). 141-146 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makino,S.,Fukuda,T.,Motojima,S.,Yukawa,T.: "Eosinophils in aivway hyperresponsiveness,in Inflamatory cells and Mediators in bronchial asthma" CRC Press, 18-249 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda, T., Akutsu, I., Amagai, M., Numao, T, Motijima, S., Makino, S.: "Antigen-induced biphasic eosinophil infiltration in the airways of actively sensitized guinea pigs and its inhibition by PAF antagonist and cyclosporin A" Jpn. Allergology. 39. 548-552 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akutsu, I., Fukuda, T., Numao, T., Makino, S.: "Inhibitory effect of FK-506 on the development of late asthmatic response and on the incereased bronchial responsiveness" Jpn. Allergology. 39. 605-609 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arima, M., Yukawa, T., Terashi, Y., Sagara, H., Makino, S.: "Involvement of platelet activating factor (PAF) in ovalbumin antigen-induced late asthmatic response and increase of airway hyperresponsiveness in a guinea pig experimental model of asthma" Jpn. Allergology. 40. 141-146 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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