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1989 Fiscal Year Final Research Report Summary

Study on the physiological roles of growth factor in reproduction.

Research Project

Project/Area Number 62440068
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionThe University of Tokyo

Principal Investigator

MIZUNO Masahiko  Dept. of Otolaryngology, University of Tokyo Professor., 医学部(病)・産婦人科, 教授 (10010175)

Co-Investigator(Kenkyū-buntansha) RYO Yoshimitsu  Dept. of Otolaryngology, University of Tokyo Assistant., 医学部(病)・産婦人科, 助手 (20220808)
ISHIHARA Satoko  Dept. of Otolaryngology, University of Tokyo Assistant., 医学部(病)・産婦人科, 助手 (30201320)
YANO Tetsu  Dept. of Otolaryngology, University of Tokyo Assistant., 医学部(病)・産婦人科, 助手 (50182390)
TSUTSUMI Osamu  Dept. of Otolaryngology, University of Tokyo Assistant., 医学部(病)・産婦人科, 助手 (60134574)
TAKETANI Yuji  Dept. of Otolaryngology, University of Tokyo Associate Professor., 医学部(病)・産婦人科, 助教授 (10114539)
Project Period (FY) 1987 – 1989
Keywordsepidermal growth factor / human endometrium / growth and differentiation of human endometrium / follicular maturation / egg maturation / endometriosis / infertility / interleukin 1
Research Abstract

Endometrial tissues undergo drastic changes during menstrual cycle. After menstruation, they proliferate and differentiate into cells with secretory activity in the preparation for egg implantation. Although sex steroids play an important role in the development of endometrial tissues, sequential events occurring in the endometrium can not be fully explained. In this study, we offer evidences that epidermal growth factor(EGF) is released from human endometrial cells and they possess the receptors for EGF. Further. EGF is shown to elicit a variety of biological actions in human endometrial cells. For instance, EGF enhances both glycogenesis and glycogenolysis, thus increasing the supply of glucose for blastocysts. We further set forth that EGF augments the capacity of progestin binding and release of prostaglandin E_2 in endometrial cells. As for the mode of action of EGF, EGF is found to phosphorylate 170 KD potein immediately after binding the cells. These findings emphasize that EGF … More may participate in the development of human endometrial tissues in concert with sex steroids, thus contributing to the acquisition of receptivity of eggs in the endometrium.
EGF-like immunoreactivity was detected in human follicular fluid, activity of which increased along with the follicular maturation. EGF is shown to stimulate the proliferation of rat granulosa cells in the presence of insulin. FSH does not affect the mitogenic action of EGF. Granulosa cells possess two kinds of EGF receptors. The addition of PSH, estradiol and testosterone resulted in an increase in the amount of EGF binding to granulosa cells. EGF stimulates the release of both progesterone and prostaglandins from cultured follicles. These stimulatory effects of EGF is synergized with phosphodiesterase inhibitor. EGF is found to induce egg maturation by increasing the activity of phosphofructokinase, a key enzyme of glucose metabolism. Hence, suggestion is made that EGF as a local factor, may be involved in the follicular development, ovulation and oocyte maturation by modulating actions of gonadotropin.
Estadiol alone has no growth-promoting effect on both endometrial and endometriotic cells. EGF stimulates cell growth of both cell types. Endometrial cells but hot endometriotic cells produce and release EGP into culture media so that stimulatory effect of exogenously added EGF is blunted in endometrial cells. Estradiol exerts its mitogenic action by enhancing the mitogenic action of EGF in endometrium. By contrast, the effect of estradiol is minimal in endometriotic cells, pointing to less dependency on estradiol for their proliferation. Progesterone inhibits cell growth of both call types in the same manner. Less

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 水野正彦: "子宮内膜症診療の現況" 産科と婦人科. 54(8). 54 (1417)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji Taketani, Masahiko Mizuno: "Cyclic changes of epidermal growth factor receptor in human endometrium daring menstrual cycle." Endocrinol Japon. 35(1). 19-25 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuji Taketani, Satoko Ishihara, Masahiko Mizuno: "Hormonal regulation of the synthesis of glycogen in caltured human endometrial cells." Human Reproduction. 233-236 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 堤治、水野正彦、内田俊也、岡孝己: "代償性腎肥大におけるepidermal growth factorの生理的意義" 日本臨床代謝学会記録. 198-199 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoko Ishihara, Yuji Taketani, Masahiko Mizuno: "Stimulatory Action of Progesterone on the Synthesis of Glycogen in Primary Cell Culture of Human Endometrium" Asia-Oceania Journal of Obstetrics and Ggnaecology.14(1). 117 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoko Ishihara, Yuji Taketani, Masahiko Mizuno: "Inhibitory Effects of Estradiol on Glycogen Synthesis in Prinary Cell Cultures of Human Endometrium" Endocrinol.Japon.35(5). 691-696 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaku S, Ishihara S, Taketani Y, Mizuno M.: "The effect of EGF on the progestin receptors in human endometrial cells." J. Fert.Implant.Japon.6. 140-143 (1989)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Taketani Y.: "Endometriosis" Aiiku54(2) 20-24, 1989.

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      「研究成果報告書概要(欧文)」より
  • [Publications] Gomibuchi H, Taketani Y, Mizuno M.: "S study on antisperm antibody in peritoneal fluid in endometriosis."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taketani Y.: "Basical and fundamental studies on biochemical properties of endometriotic tissues." Jap.J.Obstet.Gynecol. 41:971-980, 1989.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taketani Y, Ishihara S, Miyauchi A, Mizuno M.: "A role of EGF in the proliferation and differentiation of human endometrium."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi N, Taketani Y, Mizuno M: "Corupusluteum function in infertile women with endometriosis."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taketani Y, Ryo Y, Yano T, Tsutsumi O, Mizuno M: "EGF and follicular growth." Hormone and Rinsho 37:39-42.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taketani Y, Ishihara S, Miyauchi A, Mizuno M: "Biochemical aspects of implantation." Rinshofujinka sanka 44:31-36.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara S, Taketani Y, Mizuno M: "Effect of EGF on the release of prostaglandin in human endometrium."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoko Ishihara, Yuji Taketani, Masahiko Mizuno: "Stimulatory Action of Progesterone on the Synthesis of Glycogen in Primary Cell Culture of Human Endometrium." Asia-Oceania Journal of Obstetrics and Gynaecology, 14(1) : 117.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoko Ishihara, Yuji Taketani, Masahiko Mizuno: "Inhibitory Effects of Estradiol on Glycogen Synthesis in Primary Cell Cultures of Human Endometrium." Endocrinol. Japon, 35(5):691-696.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoko Ishihara, Yuji Taketani, Masahiko Mizuno: "Epidermal growth factor-like immunoreactivity in human endometrium" Asia-Oceania J. Obstet & Gynecol.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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