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1988 Fiscal Year Final Research Report Summary

Purification of chromatin proteins concerning gene expression in animals and studies on their functions

Research Project

Project/Area Number 62470117
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

MIZUNO Shigeki  Professor, Faculty of Agriculture, Tohoku University, 農学部, 教授 (90112903)

Co-Investigator(Kenkyū-buntansha) ISHIHARA Hiroaki  Assistant, Faculty of Agriculture, Tohoku University, 農学部, 助手 (50005633)
Project Period (FY) 1987 – 1988
Keywordsfibroin H- and L-chain genes / sequence-specific DNA binding protein / middle and posterior silk glands of silkworm / bent-repetitive DNA / W chromosome / W-protein / 反復塩基配列 / W染色体 / W-protein / クロマチン
Research Abstract

1. Protein factors binding to the 5'-upstream region of the fibroin light(L-) chain gene: Sequence analysis of the cosmid clone containing the fibroin L-chain gene revealed that three sequences in the 5'-upstream region, i.e, -415- -398, -306- -277, -250- -226, were highly homologous to the sequences in the -275- -155 region of the fibroin H-chain gene. It was found that protein factors which bound to the all three upstream regions were present in the 0.5M NaCl extract of the posterior silk gland nuclei of the silkworm, Bombyx mori. Particularly, proteins binding to the 64bp sequence containing the -306- -277 sequence were shown to consist of posterior silk gland-specific factor(s) and posterior and middle silk gland-common factor(s).
2. Purification and properties of W-protein: W-protein, purified from the 0.35M NaCl extract of the chicken liver nuclei, has a monomeric molecular weight of 72k but forms a multimeric form consisting of about 30 molecules, under non-denaturing conditions. W-protein binds with high affinity to the chicken W chromosome-specific, bent repetitive DNA(XhoI family). It was suggested that the XhoI family sequence wraps around the multimer of W-protein making multiple contacts through the A-T-rich minor grooves..participation of W-protein in the formation of higher-order structure of chromatin is suggested.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Fusaho Takei: J.Cell Biology. 105. 175-180 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Kodama: Chromosoma. 96. 18-25 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 水野重樹: 生化学. 59. 1308-1320 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Mizuno: Gunma Symposia on Endocrinology. 24. 27-40 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 原田昌彦: RADIOISOTOPES. 37. 243-253 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiko Harata.: J.Biological Chemistry. 263. 13952-13961 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大場義樹: "クロマチン実験法" 学会出版センター, 365 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fusaho Takei: "Further evidence for importance of the subunit combination of silk fibroin in its efficient secretion from the posterior silk gland cells" J. Cell Biology. 105. 175-180 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Kodama: "Nucleotide sequences and unusual electrophoretic behavior of the W chromosome-specific repeating DNA units of the domestic.fowl, Gallus gallus domesticus" Chromosoma. 96. 18-25 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Mizuno: "Contrastive chromatin structure of the fibroin H-chain gene during the molting cycle of the silkworm, Bombyx mori" Gunma Symposia on Endocrinology. 24. 27-40 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Harata: "Purification and characterization of W-protein. A DNA-binding protein showing high affinity for the W chromosome-specific repetitive DNA sequences of chicken" J. Biological Chemistry. 263. 13952-13961 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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