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1988 Fiscal Year Final Research Report Summary

Studies for biosynthesis, transport, secreation of glycoprotein in higher plant.

Research Project

Project/Area Number 62470119
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionNagoya University

Principal Investigator

AKAZAWA Takashi  Nagoya University, Faculity of Agriculture, 農学部, 教授 (20023400)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Hirokazu  Nagoya University, Isotope center, アイソトープセンター, 助手 (80170348)
TAKABE Tetsuko  Nagoya University, Faculity of Agriculture, 農学部, 講師 (60089852)
Project Period (FY) 1987 – 1988
Keywords-amylase / rice / oligosaccharide chair / biosynthesis / intracellular / transport
Research Abstract

Since rice seed -amylase is shown to be a typical Asn-N-linked secretory glycoprotein, the excised rice seedlings have been proved to be a feasible material to study the mechanism of the biosynthesis as well as the intracellular transport of the secretory protein in plant cells. It has been shown that -amylase molecules bearing both high mannose-type and complex-type oligosaccharide chains are secreted from the rice scutellar tissues, and a hypothetical mechanism concerning the stryctural modification of the oligosaccharide moiety linked the -amylase polypeptide chain in the golgi compartment has been proposed.
In order to further study the detailed enzymic machineries for rice -amylase biosynthesis, we have been currently employing the liquid suspension-cultured cells. Using this suspension-cultured cells, we got several data as follows.
1. Oligosaccharide structure in secreted -amylase is (GlcNAc)_2Man_3GlcNAc_2 or GlcNAcMan_3GlcNAc_2. These structures are plant specific complex type oligosaccharide.
2. Complex type oligosaccharide chain in secreted -amylase is produced from the precursor containing high-mannose type oligosaccharide chain.
3. When the oligosaccharide modification is inhibited by some environmental factors, secreted -amylase has high-mannose type oligosaccharide chain.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 赤沢堯: 現代化学・増刊10,タンパク質の細胞内輸送と膜透過. 124-135 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤沢堯: 蛋白質・核酸・酵素 別冊30号 植物の細胞生物学的研究法. 8-14 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 赤沢堯: 蛋白質・核酸・酵素 別冊30号 植物の細胞生物学的研究法. 43-47 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 三ツ井敏明: 蛋白質・核酸・酵素 別冊30号 植物の細胞生物学的研究法. 386-397 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Akazawa;T.Mitsui;M.Hayashi: The Biochemistry of Plants. 14. 465-492 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Hayashi;T.Mitsui;T.Akazawa: α-amylase mRNA in rice seed. Plant.Physiol.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Hayashi;A.Tsuru;N.Takahashi;T.Akazawa: Arch.Biochem.Biophys.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Akazawa: "Biosynthesis and secretion of enzyme in higher plant." Gendai Kakagu, zokan 10, Intracellular and membrane transport of protein. 124-135 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Akazawa: "Studies of plant organella" Tanpakushitsu kakusankouso, bessatsu 30, Cell Biology of Plants. 8-14 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Akazawa: "Golgi apparatus" Tanpakushitsu kakusan kouso, bessatsu30, Cell Biology of Plants. 43-47 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Mitsui: "Biosynthesis of secretory enzyme" Tanpakushitsu kakusan kouso 30, Cell Biology of Plants. 386-397 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Akazawa, T. Mitsui and M. Hayashi: "Recent progress in -amylase biosynthesis" The Biochemistry of Plants. 14. 465-492 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Hayashi, T. Mitsui and T. Akazawa: "Regulatory role of Ca2t on the level of -amylase mRNA in rice seed" Plant.Physiol.Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Hayashi, A. Tsuru, N. Takahashi and T. Akazawa: "Biosynthesis and intracellular transport of -amylase in cultured cells of rice seed" Arch. Biochem. Biophys.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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