1988 Fiscal Year Final Research Report Summary
Aerial mycelium-inducing substances of actinomycetes and their application to the regulation of antibiotic production
| Project/Area Number |
62470125
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | Nagoya University |
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Principal Investigator |
MARUMO Shingo Department of Agriculture, Nagoya University, 農学部, 教授 (30023394)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Actinomycetes / Aerial mycelium / Pamamycin / Calcium ion / 抗生物質生産 / カルシウムイオン |
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| Research Abstract |
As the results of screening experiments on actinomycetes in whcih aerial mycelium formation was regulated by active substances present in their cultured materials, we found that several species produced aerial mycelium-inducing substances. The active substance named pamamycin-607 was isolated from Strepomyces alboniger IFO 12738, and the structure was elucidated as a novel sixteen-membered macrodiolide with dimethylamino group-bearing side chain. At 0.1 ug/disc pamamycin-607 induced aerial mycelia in the aerial mycelium-negative strain. When KMnO_4 was partitioned with pamamycin-607 between benzene and water, MnO_4- but no K^+ was partitioned transferred from the water to the benzene layer; thus pamaycin-607 was shown to be a novel anion-transfer substance. From the culture broth of S. ambofaciens the actice substance was purified and identified as Ca(OAc)_2. It was assumed that the Ca^<2+> was derived from the CaCO_3 which was a component of the medium and had been solubilized as S. ambofaciens grew and acidified the culture medium. Ca^<2+> induced aerial mycelium formation in S. ambofaciens at above 1 mM, and its effect was inhibited by ECTA, a Ca^<2+> specific chelating reagent. A survey of 36 strains of actinomycetes showed that Ca^<2+> regulated aerial mycelium formation in 21 straing (58%) of them. The Ca^<2+> concentration required for aerial mycelium formation in the culture medium ranged from 0.1 to 1.5 mM. We found that S. venezuelae produced the aerial myceliuminducing substance; which was water-soluble and not adsorbed on charcoal. Interestingly, when aerial mycelium was induced by thiscompound, the strain began to produce the antibiotic.
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