• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1988 Fiscal Year Final Research Report Summary

Molecular genetic study on gene expression in chloroplasts.

Research Project

Project/Area Number 62480001
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionKyoto University

Principal Investigator

OZEKI Haruo  Faculty of Science, Kyoto University, 理学部, 教授 (50028106)

Co-Investigator(Kenkyū-buntansha) INOKUCHI Hachiro  Faculty of Science, Kyoto University, 理学部, 助教授 (20028195)
Project Period (FY) 1987 – 1988
KeywordsLiverwort chloroplasts / Chloroplast gene expression / Prokaryotic basic genes / Catalytic introns / RNA processing / Ordered splicing / トランス・スプライシング
Research Abstract

Chloroplast genes are expressed within the prganelles via their own machinery for prttein biogenesis, although the machinery itself is composed not only of their own gene products but also of the nuclear gene products. We have determined the entire DNA sequence of liverwort chloroplast genome and deduced 127 distinct genes. About half of them are concerned with the basic mechanisms of gene expression in the chloroplasts, such as transcription and translation, and share many features with prokaryotic organisms. Chloroplast ribosomes are prokaryotic 70S, and genetic signals such as promotors, terminatews, SD-sequences etc. are also bacteria types. Thus the genetic system of chloroplasts is basically prokaryotic, supporting the hypothesis of endosymbiotic origin of bacteria. On the other hand, however, many chloroplast genes are interruped by introns(catalytic ones of hroup I or II), differring from bacteria, and thus require post-transcriptional RNA splicing for their gene expression. Analysis of chloroplast RNAs prepared from cultured liverwort cells showed that the primary RNA transcripts usually contained poly-cistronic messages which were then processed into smaller mRNAs prior to splicing. If a gene contained two introns, the splicing proceeded successively from the 5' to 3' direction. We also proposed a bimolecular interaction model for trans-splicing. In conclusion, we postulated matulation pathways for mRNAs in chloroplasts from the primary RNA transcripts via RNA processing and splicing cis and trans.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] H.,Ozeki;et al.: Cold Spring Harbor Symposia on Quant.Biol.52. 791-804 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.,Ohyama;et al.: J.Mol.Biol.203. 281-372 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.,Kohchi;et al.: Current Genetics. 14. 147-154 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.,Kochi;et al.: Nucl.Acids Res.16. 10025-10036 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.,Ozeki,et al.: Genome. 31. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.,Michel;et al.: Gene. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Ozeki et al.: "Genetic system of chloroplasts." Cold Spring Harbor Symp. Quant. Biol.52. 791-804 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Ohyama et al.: "Structure and organization of Marchantia polymorpha chloroplast genome. I - IV." J. Mol. Biol.203. 281-372 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kohchi et al.: "Ordered processing and splicing in a polycistronic transcript in liverwort chloroplast." Current Genetics. 14. 147-154 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Kohchi et al.: "A nicked group II intron and trans-splicing in liverwort, Marchantia polymorpha, chloroplasts." Nucleic Acids Res.16. 10025-10036 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Ozeki et al.: "The chloroplast genome of plants - A unique origin." Genome. 31. (1989)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1990-03-20  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi