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1988 Fiscal Year Final Research Report Summary

Molecular population-genetic studies on adaptive evolution of Drosophila population.

Research Project

Project/Area Number 62480003
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionKyushu University

Principal Investigator

MUKAI Terumi  Professor, Faculty of Science, Kyushu University, 理学部, 教授 (30091242)

Co-Investigator(Kenkyū-buntansha) KUSAKABE Shinichi  Assistant, Faculty of Science, Kyushu University, 理学部, 助手 (40153275)
HARADA Ko  Assistant, Faculty of Science, Kyushu University, 理学部, 助手 (40150396)
Project Period (FY) 1987 – 1988
KeywordsViability polygenes / Insertion-deletion / Beneficial mutations / Transposon / Transposition rate of transposon / Copia / Hobo / hobo
Research Abstract

To understand the molecular basis of adaptive evolution, four experiments were conducted and the following results were obtained: 1. We have reported that there is a north-to-south cline of additive genetic variance for viability and that the excess in the southern population has been maintained by genotype-environment interaction. To obtain the molecular basis, southern blot analysis was conducted for the Ogasawara (south) and Aomori (north) populations. Forty-three second chromosomes were analyzed for each population using four proves inclusing the Gpdh, Adn, Amy and Pu genes, with eight restriction enzymes. Significant difference was not detected between the northern and southern populations except the following; in the southern population, the number of unique insersion/deletions was significantly larger than that in the northern population. Thus, insertions/deletions may be one of the important condidates of viability polygenes. 2. Duplication and triplication of the sn glycerol-3-phosphate dehydrogenase gene (Gpdh) were detected in natural populations. The former were found to be polymorphic in every population tested. The latter were polymorphic only in the Aomori and Ogasawara populations. 3. Using the previous finding of the age of In(2L)t in the Raleigh, N.C. population, search for beneficial mutations was conducted. It was detected that some beneficial mutation occurred in this inversion about 4000 generations ago and that its frequency has increased to 18% in the In(2L)t-carrying chromosomes. 4. The transposition rates, both the exision and insertion rates, were estimated for copia, copia-like elements (17,6, 412), hobo and I__- elements. Copia and copia-like elements are not cross-mobilized with the movement of hogo elements under their dysgenic condition.Furthermore, it was found that the mutator factor of Yamaguchi and Mukai (1974) is hobo elements.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Koga,A.: Submitted to Genetics. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takano,T.: Accepted by Proceedings of the National Academy of Science,USA. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada,K.: Proceedings of the Japan Academy. 64(B). 193-196 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koga,A.: Proceedings of the Japan Academy. 64(B). 9-12 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nitasaka,E.: Prodeedings of the National Academy of Science,USA. 84. 7605-7608 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi,O.: Japanese Journal of Genetics. 62. 205-216 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koga,A.: "The genetic structure of natural populations of Drosophila melanogaster. XXII. Effect of beneficial mutation." Submitted to Genetics. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takano,T.: "Polymorphism for the number of tandemly multiplicated glycerol-3-phosphate dehydrogenase genes in Drosophila melanogaster." Accepted by Proceedings of the National Academy of Science, USA. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harada,K.: "A new family of mobile dispersed middle repetitive elements in Drosophila melanogaster." Proceedings of the Japan Academy. 64(B). 193-196 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koga,A.: "Wide-spread polymorphism of a tandem duplication in the region of the glycerol-3-phosphate dehydrogenase gene in Drosophila melanogaster." Proceedings of the Japan Academy. 64(B)No.1. 9-12 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nitasaka,E.: "Repressor of P elements in Drosophila melanogaster: Cytotype determination by a defective P element carrying only open reading frames 0 through 2." Proceedingd of the National Academy of Science, USA. 84. 7605-7608 (1987)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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