1988 Fiscal Year Final Research Report Summary
Studies of genetic transformation in cereal plants
| Project/Area Number |
62480030
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Breeding science
|
|---|
| Research Institution | Institute of Biological Sciences, University of Tsukuba |
|---|
Principal Investigator |
UCHIMIYA Hirofumi Institute of Biological Sciences, University of Tsukuba, 生物科学系, 助教授 (50142229)
|
|---|
| Project Period (FY) |
1987 – 1988
|
| Keywords | Rice / Transformation / Electroporation / DNA |
|---|
| Research Abstract |
Rice plants were regenerated from protoplants treated with plasmid DNA possessing a chimeric gene encoding aminoglycoside phoshotransferase II (APH(3')II) and CaMV35S promoter. Rice protoplants isolatied from suspension cells were treated by electroporation or PEG in the presence of plasmid DNA. The planting efficiency of electroporated protplasts was about 0.5% after three weeks. The dividing cells were selected G418-resistant colonies formed in the control.
Calli larger than l mm were transferred to regeneration medium. Severl plants were regenerated from drug resistant calli. Leaves of these plants were not bleached when grown in the medium containing 50 ug/ml Kanamycin sulphate APH(3')II activity was detected in the leaf extracs of transformed plants. Analysis of DNA of the trasgenic plants and progenies indicated the presence of indicated the presence of intact forms of foreign DNA in recipient plants.
Other exampl of DNA transformation resulted in the production of transgenic plants will be pressented. Efforts are being made to visualize the manner and the sites of foreign DNA integration in rice plants.
|
