• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1988 Fiscal Year Final Research Report Summary

Functional interconnection between cholinergic and peptidergic neurons in bullfrog sympathetic ganglia

Research Project

Project/Area Number 62480109
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionKurume University

Principal Investigator

AKASU Takashi  Kurume University School of Medicine Professor, 医学部, 教授 (60113213)

Co-Investigator(Kenkyū-buntansha) TOKIMASA Takayuki  Kurume University School of Medicine Associate Professor, 医学部, 助教授 (50155511)
Project Period (FY) 1987 – 1988
KeywordsModulation of synaptic transmission / Cholinergic neuron / Peptidergic neuron / Synaptic interaction / Sympathetic ganglia / Bullfrog / 受容体感受性促進 / シナプス伝達調節
Research Abstract

Pharmacological study has reported previously modulation of cholinergic transmission induced by interaction between nicotinic and peptidergic transmitter substances. However, there was considerable disagreement among the experimental findings. The purpose of the present study was to examine the existence of synaptic interaction between peptidergic and nicotinic postsynaptic responses in bullfrog sympathetic ganglia, using intracellular and voltage-clamp recording methods. Stimulations of both 3rd-5th (0.2 Hz) and 8th (30 Hz) spinal nerves evoked the fast excitatory postsynaptic potential (EPSP) superimposed with the late slow EPSP at the same sympathetic neuron. The amplitude of the fast EPSPs was decreased during the course of the late slow EPSP in a majority of neurons. The quantal content of the fast EPSP was also decreased. Acetylcholine-induced depolarization ACh potential and current (ACh current) produced by ionophoretic application of ACh were not changed or increased during th … More e late slow EPSP. Bath-application of luteinizing hormone-releasing hormone (LH-RH; 40 nM-4 M) depressed the fast EPSP in a concentration-dependent manner. At a concentration 1 M, LH-RH produced a 63 8 % depression of the quantal content of the fast EPSP. LH-RH depressed the frequency of the miniature EPSP. Antagonists for LH-RH receptor ([D-Phe^<2,6>, Pro^3]-LH-RH and [d-pGlu^1, D-Phe^2, D-Tri^<3,6> -LH-RH) prevented the presynaptic inhibition of the fast EPSP induced by LH-RH. These results suggest that the fast EPSP is depressed during the late slow EPSP by decreasing the evoked release of ACh from presynaptic nerve terminals in bullfrog sympathetic ganglia. In some sympathetic B neurons. LH-RH increased the ACh current and shifted the dose response curve in parallel to the left. Voltage-clamp analyses using a single sympathetic neurons showed that LH-RH did not change the properties of ion channels associated with the nicotinic receptor. LH-TH may facilitate the sensitivity of the nicotinic ACh receptors by increasing an affinity of ACh to the receptor. Less

  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Akasu,T.: Brain Research. 405. 375-379 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] North,R.A.: Journal of Physiology. 386. 333-353 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsurusaki,M.: Neuroscience Letters. 77. 303-307 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akasu,T.: British Journal of Pharmacology. 91. 453-455 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Galligan,J.J.: Neuroscience Letters. 82. 167-171 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ariyoshi,M.: Brain Research. 435. 241-248 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akasu,T.: Pflugers Archiv European Journal of Physiology. 411. 80-87 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishimura.T.: Brain Research. 442. 399-402 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hasuo,H.: Kurume Medical Journal. 35. 43-48 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akasu,T.: Neuroscience Letters. 92. 270-274 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akasu,T.: Comparative biochemistry and Physiology(Pt.C). 91. 241-246 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hasuo,H.: Pflugers Archiv European Journal of Physiology. 413. 206-208 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koketsu,K.: "Presynaptic Regulation of Neurotransmitter Release" Freund Publishing House Ltd., inpress (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akasu,T.: "Central Cholinergic Synaptic Transmission" Birkhauser Verlag AG, inpress (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akasu, T.: "Evidence for epinephrine-induced depolarization in neurons of bullfrog sympathetic ganglia" Brain Research. 405. 375-379 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ariyoshi, M.: "Voltage-clamp studies of the inhibition of -aminobutyric acid response by glucocorticoids in bullfrog primary afferent neurons" Brain Research. 435. 241-248 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishimura, T.: "5-Hydroxytryptamine inhibits cholinergic transmission through 5-HT_<1A> receptor subtypes in rabbit vesical parasympathetic ganglia" Brain Research. 442. 399-402 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasuo, H.: "Facilitation of nicotinic acetylcholine responses during the late slow EPSP in a subpopulation of bullfrog sympathetic neurons" Kurume Medical Journal. 35. 43-48 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akasu, T.: "5-Hydroxytryptamine facilitates GABA-induced depolarization in bullfrog primary afferent neurons" Neuroscience Letters. 92. 270-274 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akasu, T.: "Postsynaptic modulation of cholinergic transmission by endogenous substances" Comparative Biochemistry and Physiology (Pt.C). 91. 241-246 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasuo, H.: "Presynaptic inhibition of cholinergic transmission by peptidergic neurons in bullfrog sympathetic ganglia" Pflugers Archiv European Journal of Physiology. 413. 206-208 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasuo, H.: "Peptidergic inhibition of cholinergic transmission in bullfrog sympathetic ganglia" Japanese Journal of Physiology. 38. 643-658 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akasu, T.: "Adrenaline inhibits muscarinic transmission in bullfrog sympathetic ganglia" Pflugers Archiv European Journal of Physiology. in press. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koketsu, K.: Presynaptic Regulation of Neurotransmitter Release. Freund Publishing House Ltd., (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akasu, T.: Central Cholinergic Synaptic Transmission. Birkhauser Verlag AG, (1989)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1990-03-20  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi