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1988 Fiscal Year Final Research Report Summary

Development of Molecular Pharmacology in relation to Calcium Binding Proteins

Research Project

Project/Area Number 62480117
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionThe University of Tokyo

Principal Investigator

NONOMURA Yoshiaki  The University of Tokyo, Faculty of Medicine, Professor, 医学部, 教授 (80009993)

Project Period (FY) 1987 – 1988
KeywordsCalcium binding protein / Smooth muscle phosphorylation / Inhibitor of phospherylation / Purealin / K-252a / Amilormide / Okadic acid / Reserpine / カルミジェン拮抗薬
Research Abstract

There is calmodulin in smooth muscle as calcium binding protein which is neussary for phosphorylation of myosin light chain. On the other hand, recase of neurotransmitter requires Ca^<2+> and calcium bindmg protein plays an important role of it. Using drugs and factors affecting Ca^<2+> binding proteins, I studied the change of these Ca^<2+> binding proteins and theis function.
1) Puredlin; it was preparad from sea sponge and acted on myosin ATPase for the effect to phosphorylated state. In this study. it acts on calcium calmodulin interaction as an imhibitor. 2) K-252a; it is a kind of bactersal product and antibiotics. This chemicul has an effect to the inhibitory action to smooth muscle myosin light chain kinase at low conuntrations and to calium calmodulin interaction at high concentrations. 3) Amiloride; this diuretic drug inhibits contraction of smooth muscle in intact and skinned state. These effects were dednced from the inhibition of phosphorylation which induced by speiralizad inhibition of light chain kinase. 4) Okadic acid; it was extracted from sea sponge and induced contraction of smooth muscle in free Ca^<2+> and the presence of Ca^<2+> blocker. It acts as an activator of light chain kinase with an inhibitor of phasphatase. 5) Reserpine; it is an activator of actin polymerization and it binds to Go actin.
30KD Ca^<2+> binding protein was purified from bovine cerebellum. Its novel protin may have an impatant role in neurotrausmitlering antion.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] J.Takito;Y.Ohizumi;H.Nakumura;K.Ebisawa;Y.Nonomura: European Journal of Pharmacology. 142. 189-195 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Ozaki;T.Kojima;H.Karaki;K.Kohama;Y.Nonomura: Journal of Pharmacology and Experimental Therapeutics. 243. 370-377 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Ozaki;K.Kohama;Y.Nonomura;S.Shibata;H.Karaki: Journal of Pharmacology and Experimental Therapeutics. 243. 1167-1173 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Nakanishi;H.Kase;S.Nakamura;Y.Nonomura: Journal of Biological Chemistry. 263. 6215-6219 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Nakamura;K.Ohmi;Y.Nonomura: Molecular Pharmacology. 33. 604-610 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Kurokawa;Y.Nonomura: Journal of Biochemistry. 103. 8-10 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野々村禎昭: "Ca^<2+>とアクチンフィラメントの重合・脱重合 カルシュウムイオンと細胞機能" 共立出版, 1-485 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Takito;Y.Ohizumi;H.Nakamura;K.Ebisawa;Y.Nonomura: "The mechanism of inhibition of light chain phosphorylation by purealin in chicken gizzard myosin" European Journal of Pharmacology. 142. 189-195 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Ozaki;T.Kojima;H.Karaki;K.Kohama;Y.Nonomura: "Inhibition by amiloride of contractile elements in smooth muscle of guinea pig tesnia coli and chicken gizzard" Journal of Pharmacology and Expermeutal Therapeutics. 243. 370-377 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Ozaki;K.Kohama;Y.Nonomura;S.Shibata;H.Karaki: "Calium independent phosphorylation of smooth musele myosin light chain by okadic acid isolated from black sponge" Journal of Pharmacology and Experimental Therapeutics. 243. 1167-1173 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Nakanishi;H.Kase;S.Nakamura;Y.Nonomura: "K-252a, a novel microvial product, inhibits smooth muscle myosin light chain kinase" Journal of Biological Chemistry. 263. 6215-6219 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Nakamura;K.Ohmi;Y.Nonomura: "Low concentiation of reserpine accelerutes actiu polymerization via interaction with G-actin" Molecular Pharmucology. 33. 604-610 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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