1988 Fiscal Year Final Research Report Summary
Immunopathological approach to pathogenesis of human idiapathic spermatogenic disorder
| Project/Area Number |
62480137
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
HATAKEYAMA Shigeru Tokyo Medical and Dental University, Medical Faculty Proffessor, 医学部, 教授 (30045926)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TERADA Takahiko Tokyo Medical and Dental University, Medical Faculty Ass. Proffessor, 医学部, 助手 (90197799)
EISHI Yoshinobu Tokyo Medical and Dental University, Medical Faculty Ass. Proffessor, 医学部, 助手 (70151959)
|
|---|
| Project Period (FY) |
1987 – 1988
|
| Keywords | Spermatogenic disorder / Autoimmunity / Anti-Sertoli cell autoantibody / 精細管基成膜 |
|---|
| Research Abstract |
Human autoantibody to Sertoli cells was detected in normal human sera. This IgM-type autoantibody was undetectable during neonatal period and was found in 11.5% of 365 serum samples taken from adult healthy persons of both sexes. This human autoantibody to Sertoli cells exhibited quite the same target-organ specificity and:multi-organ reactivity as those of the murine monoclonal autoantibody(IgM class) to Sertoli cells (TM-1: WHO registered code T43) which has already been demonstrated capable of inducing experimental spermatogenic disturbance in mice. These human and murine autoantibodies could recognize testicular self-antigens of molecular weight 67,000 and 23,000 in Sertoli cells.
Monoclonal antibody to basement membrane of the seminiferous tubules (TM-2: WHO registered code T44) allowed the autoantibody to reach the Sertoli cells across the blood-testis barrierr. Autoantibody with the same target-tissue specificity as TM-2 was also demonstrated in human sera, both recognizing type-IV collagen molecules as target self-antigens of the testis.
Thus, human spermatogenic disturbance could be easily induced even in healthy individuals, once a functional impairment of Sertoli cells and blood-testis barrierr composed mainly of basement membrane of seminiferous tubules were induced by either these naturally-occuring autoantibodies or other toxic damages of blood-testis barrierr.
|
Research Products
(14 results)
