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1988 Fiscal Year Final Research Report Summary

Analysis of biological roles of class II major histocompatibility antigen by anti-sense RNA

Research Project

Project/Area Number 62480163
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionKobe University

Principal Investigator

TOKUHISA Takeshi  Kobe University School of Medicine・Professor, 医学部, 教授 (20134364)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Masaru  Chiba University School of Medicine・Professor, 医学部, 教授 (80110310)
Project Period (FY) 1987 – 1988
Keywordsclass II major hitocompatibility antigen / anti-sense RNA / Gene transfection / Protoplast method / antigen presenting activity
Research Abstract

We have established an anti-sense RNA system which is capable of regulating the expression of the endogenous class II MHC (la) molecule in order to elusidate the role of la molecules on immune responses. The 100 base pairs of the l-A chain gene were constructed under the control of a metallothionein gene promoter in an anti-sense orientation. This anti-sense gene was taransfected into the la^+ B lymphoma cells. The amount of la molecules on the transfectants was specifically reduced to less than 5% of the control level. The rations of the amount of anti-sense and sense mRNA in the transfectants correlated with the amount of the la molecules, indicating that the duplex fomation of sense and anti-sense RNA inhibits translation of the sense mRNA. Antigen presenting cell activities of the transfectants to the la restricted T cells also correlated with the amount of la molecules.
simultaneously, this anti-sense gene was microinjected into fertilized eggs of C57BL/6xSJL F1 mice to raise the transgenic mice. In these transgenic mice, 1)the amount of la molecules on the spleen cells was distinctly reduced, 2) anti-sense rna was detected in total RNA from spleen cells of the transgenic mice. We are examining immune abnormalities of these mice.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Uchiyama,K.;et al.: Hybridoma. 6. 845-654 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ruther,U.;et al.: Cell. 53. 847-856 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hatano,M.;et al.: Int. Immunol.(1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 幡野雅彦 他: 日本免疫学会誌. 18. 752 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 徳久剛史 他: 日本免疫学会誌. 18. 753 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 徳久剛史 他: 日本分子生物学会誌. 11. 173 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taniguchi,M.;et al.: "Antigen-specific suppressor molecules produced by T cells.In Handbook of experimental Immunology." Blackwell Scientific Publications, 11 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokuhisa,T.;et al.: "Specific inhibition of the major histocompatibility complex(MHC)expression by anti-sense RNA." Proceedings of the 7th seminar on Science and Technology"Molecular Biology", 14 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchiyama,K.; Saito,H.; Tokuhisa,T.; Imai,K.; Taniguchi,M.: "High frequency of loss of human kappa light chain expression in mouse-human hetero hybridomas." Hybridoma. 6. 645-654 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ruther,U.; Muller,W.; Sumida,T.; Tokuhisa,T.; Rajewsky,K.; Wagner,E.F.: "c-fos expression interferes with thymus development in transgenic mice." Cell. 53. 847-856 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hatano,M.; Aizawa,S.; Soejima,T.; Shigemoto,K.; Taniguch,M.; Tokuhisa,T.: "Specific regulation of class II MHC gene expression by anti-sense RNA." Int. Immunol.(1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokuhisa,T.; Hatano,M.: Specific inhibition of the major histocompatibility complex (MHC) expression by anti-sense RNA.Proceedings of the 7th seminar on Science and Technology "Molecular Biology", 14 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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