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1988 Fiscal Year Final Research Report Summary

Clarification of etiology of neonate and infant vitamin K deficiency

Research Project

Project/Area Number 62480173
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Hygiene
Research InstitutionKyoto University

Principal Investigator

KIMURA Mieko  Faclty of Medicine Associate Professor, 医学部, 助教授 (60025658)

Co-Investigator(Kenkyū-buntansha) YOKOI Katsuhiko  Faclty of Medicine Reserch Associate, 医学部, 助手 (10200883)
ITOKAWA Yoshinori  Faclty of Medicine Professor, 医学部, 教授 (80025593)
Project Period (FY) 1987 – 1988
Keywordsvitamin K / vitamin K deficiency / neonate / infant / determination method for vitamin K / platinum Column / plasma / umbilical cord plasma / breast-milk / qmniotic membrane / amniotic fluid / meconium
Research Abstract

It is well known that neonatal and infantile vitamin K deficiency causes melena neonatorumand intracranial hemorrhagic disorders. These disorders occur more frequently in breast-fed babies than in bottle fed babies, but the reason is still obscure. There have been some suggestions as to how to prevent babies from having vitamin K deficiency. On the other hand, determination of endogenous concentrations of the K vitamins (phylloquinone:K1 and menaquinones:MK-4, MK-l, MK-7)in human plasma is very difficult. In this study, at first we report clear separations and determination methods of vitamins K1 and MK-4, MK-l and MK-7 in placenta with the use of thin layer chromatography, followed by high performance liquid chromatography(HPLC) and fluorometric determination after post-column chemical reduction, in maternal and umbilical cord plasma by HPLC and fluorometric determination follwed post-column electochemical reduction in maternal and umnilical cord plasma by HPLC and fluorometric determ … More ination follwed platinum reduction column. Next, we describe the availability of vitamin Ks in different tissues. Vitamins K1 and MK-4, MK-6 and MK-7 were found in placenta, and maternal plasma, much K1 and MK-48 and slightly MK-l and MK-7 were found in breast milk and amniotic membrance, and K1 and MK-4 were found in umbilical cord plasma, smniotic luid, meconium and vernix caseosa. Third, to prevent early hemorrhagic diseases of the newborn, we have administered vitamin K2 drug by i.v. or oral route to mother before delivery, and vtaimin Ks concentrations in umbilival cord plasma, maternal plasma at birth and breast milk of 5 days after delivery were found to be siginficantly high as ccompared with those of non-treated mothers group. Therefore, oral or i.v. administration of vitamin Ks drug to mother before delivery which causes early and continuos transportation of vitamin Ks from mother to neonate is safer and more preferable than intramusclar injection to neonate, whic involves a certain degree of danger. Less

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] H.,Hiraike;M.,Kimura;Y.,Itokawa: Amer.J.Obestet.Gynecol.158. 564-569 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.,Hiraike;M.,Kimura;Y.,Itokawa: J.Chromatogr. 430. 143-148 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平池秀和,木村美恵子,糸川嘉則: 微量栄養素研究. 5. 115-119 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平池秀和,木村美恵子,糸川嘉則: ビタミン. 63. 25-28 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平池秀和,横井克彦,関根健二,S.M.Ahmed,佐田文宏,木村美恵子,糸川嘉則: マグネシウム. 7. 9-14 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平池秀和,関根健二,S.M.Ahmed,佐田文宏,木村美恵子,糸川嘉則: マグネシウム. 6. 45-50 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Hiraike; M. Kimura; Y. Itokawa: "Pistribution of K vitamins(phylloguinone and menaguinones) in hunan plsceuta and maternal and umbilical cord plasma." Amer. J. Obstet Gynecol.158. 564-569 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hiraike; M. Kimura; Y. Itokawa: "Determination of K vitamins (phylloguinone and mehaguinones) in umbillical cord plasma by a platinum reduction column." J. Chromatogr.430. 143-148 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hiraike; K. Sekine; S. M. Ahmed; F. Sata; M. Kimura; Y. Itokawa: "Concentrations of magnesium and calcium in human placentae and in maternal and umbillical cord plasma." Magnesium. 6. 45-50 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hiraike; M. Kimura; Y. Itokawa: "Measurement of K vitamins in human placeuta by high-performance liguid chromatography with flusrometric deteetion" Jpn. J. Hyg.41. 764-768 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Hiraike; M. Kimura; Y. Itokawa: "Administration of vitamin K_2 syrup to mather before delivery" Vitamins. 63. 25-28 (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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