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1988 Fiscal Year Final Research Report Summary

Molecular and Cell Biological Studies on Early Lymphocytes Development.

Research Project

Project/Area Number 62480231
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionThe National Children's Hospital

Principal Investigator

KOBAYASHI Noboru  The National Children's Hospital, 小児医療研究センター, 院長 (50009916)

Co-Investigator(Kenkyū-buntansha) 中山 雅之  国立小児病院小児医療研究センター, 研究員 (00165771)
MIZUTANI Shuki  Department of Virology, The National Children's Research Center, 室長 (60126175)
秦 順一  国立小児病院小児医療研究センター, 部長 (90051614)
NAKAYAMA Masayuki  Department of Immunology, The National Children's Research Center
HATA Jun-Ichi  Department of Pathology, The National Children's Research Center (90051614)
Project Period (FY) 1987 – 1988
KeywordsLymphocyte / Common ALL Antigen / Immunoglobulin Gene / 胸腺上皮細胞
Research Abstract

In order to study the molecular mechanisms for early lymphocyte development, we have carried out the biochemical analysis of Common ALL antigen, which is expressed on the early stage of differentiation of B /or T lymphocyte. The result of our study indicates that the minor difference of CALLA molecule among differnt tissues is mainly due to those of syalidization.
In order to clarify the correlation of the differentiation antigen expression and genome organization of ig heavy chain gene, we have studied the molecular features in 78 ALL patients. As the results of this study, we found B precursor ALLs with germ line configuration of IgH gene, those with the rearrangement of Domega52 and those with the rearrangement of D 5' to Domega52. We propose a model for the classification of early B lymphocyte based upon these findings. Thymic epithelial cell lines were established by using gene transfer technique. SV40 ori- gene was introduced into fetal thymic epithelia. As the results 2 cell lines were established. They produced M-CSF and expressed LFA3 molecule which is a lignad for CD2.
Our study is expected to help disclose the mechanisms for lymphocyte development in human.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] M.Ozaki;S.Mizutani;N.Kobayashi: Leukemia in Press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Nakamura;S.Mizutani;N.Kobayashi: Leukemia in Press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Mizutani et al.: Proc.Natl.Acad.Sci.USA. 84. 4999-5003 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Fujimoto;J-I Hata: Virchows Archive A Pathol Anat Histopathol. 412. 307-314 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.Fujimoto;J-I Hata: Hybridoma. 7. 227-236 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Nonoyama;M.Nakayama: Eur J.Immunol.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Ozaki; S. Mizutani; N. Kobayashi;et al.: "Molecular Analysis of 5'J Region of Immunoglobulin Heavy Chain Gene in Human Acute Leukemia." Leukemia.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Nakamura;S.Mizutani; N. Kobayashi: "MOLECULAR Diversity of Precursor B Acute Lymphoblastic Leukemias Identified by the Immunoglobulin Heavy Chain Gene organization." Leukemia.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Mizutani;et al.: "Cloning of Human Thymic Subcapsular Cortex Epithelial Cells with T lymphocyte Binding Site and Hemopoietic Activity." Proc Natl Acad. Sci USA. 84. 4999-5003 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Fujimoto; J-I Hata: "Ki-1 lymphoma in Childhood; Immunocytochemical Analysis and Significance of Epithelial Membrane Antigen as a New Marker." Virchows Archive A Pathol Anat Histopatholo. 412. 307-314 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Fujimoto; J-I Hata: "Immunological and Immunodhemical Analysis on Common Acute Lymphoblastic Leukemia Antigen(CALLA); Evidence that CALLA on ALLs and Granulocytes are Structurally Related." Hybridoma. 7. 227-236 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20   Modified: 2017-04-05  

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