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1988 Fiscal Year Final Research Report Summary

Research for the pathogenesis of type III hyperlipidemia by the method of molecular biology

Research Project

Project/Area Number 62480251
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionKyoto Univercity

Principal Investigator

YOSHIKATSU Nakai  Kyoto University, 医学部, 講師 (10115892)

Project Period (FY) 1987 – 1988
KeywordsApolipoprotein E / Point mutation / Hyperlipidemia / DNA amplification / Polymerase Chain Reaction / synthetic oligonucleotide / RNA probe / 遺伝子型
Research Abstract

A rapid procedure for determing apolipoprotein E genotype grom genomic DNA has been deceloped.
1) Single base substitutions can be detected and localized by a simple and rapid method that involves ribonuclease cleavage of single base mismatches in RNA:DNA heteroduplexes. The feasibility of this method for localizing base substitutions directly in genomic DNA samples is demonstrated by the detection of single base mutations in DNA obtained from individuals with type III hyperlipidemia, a genetic disorder in apolipoprotein E gene expression.
2) DNA is amplified by the method for in vitro DNA amplification (Polymerase Chain Reaction). Two sets of oligonucleotides were synthesized and used to discriminate either between epsilon3 and epsilon4 alleles of between epsilon3 and epsilon2 alleles. Combination of the allele-specific oligonucleotide hybridization with the method for in vitro DNA amplification dramatically improved the sensitivity and the releability of the procedure.
Adaptation of a simple strategy involving direct coloning and DNA sequencing of in vitro amplified DNA enables rapid identification of any mutation within the apo E gene area encoding the receptor binding domain.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 妹尾圭子,中井義勝,塚田俊彦 他: 動脈硬化.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 妹尾圭子,中井義勝,塚田俊彦 他: 動脈硬化.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 妹尾圭子,中井義勝,塚田俊彦 他: 動脈硬化.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.,Senoo;Y.,Nakai;T.,Tsukada;et al: Atherosclerosis.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.,Nakai;K.,Senoo;H.,Kawamura;et al: Arteriosclerosis.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.,Nakai;T.,Koh;T.,Tsukada;et al: Metabolism.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.,Tsujii;Y.,Nakai;J.,Fukata;et al: Peptides. 8. 1075-1078 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.,Tsujii;Y.,Nakai;J.,Fukata;et al: Endocrinol.Japon.34. 903-909 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.,Usui;Y.,Nakai;T.,Tsukada;et al: Mol.Endocrinol.2. 871-875 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.,Nakai;T.,Tsukada;T.,Usui;et al: Prog.in endocrinol.edited by Imura,H. et al. 737-742 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.,Tsujii;Y.,Nakai;H.,Takahashi;et al: Brain Res.475. 371-375 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中井義勝,辻井悟,深田川順一 他: 日本肥満学会. 102-104 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakai, Y.: "Apolipoprotein E polymorphism and hyperlipidemia." Arteriosclerosis.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Senoo, K.: "Rapid diagnosis of apolipoprotein E isoform by a new method of DNA analysis." Atherosclerosis.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakai, Y.: "Pathogenesis of hypercholesterolemia in anorexia nervosa." Metabolism.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Usui, T.: "Expression of adrenocorticotropin-releasing hormone (CRH) precursor gene in placenta and other non-hypothalamic tissues in man." Mol. Endocrinol.2. 871-875 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsujii, S.: "Effect of food deprivation on regional brain glucose utilization in lean and fatty Zucker rats." Brain Res.475. 371-375 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakai, Y.: Proopiomelanocortin (POMC) synthesis in the pituitary and non-pituitary tissues.Elsevier Science Publishers B.V., 737-742 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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