1988 Fiscal Year Final Research Report Summary
Pathophysiology and molecular genetics of familial cortisol resistance
| Project/Area Number |
62480254
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
内分泌・代謝学
|
|---|
| Research Institution | Osaka University(Medical School) |
|---|
Principal Investigator |
MORIWAKI Kaname Osaka University Medical School Associate Professor, 医学部, 助教授 (90028548)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAJIMA Koji Osaka University Medical School Research Assistant, 医学部, 助手 (60188240)
IIDA Sayomi Osaka University Medical School Research Assistant, 医学部, 助手 (40159554)
|
|---|
| Project Period (FY) |
1988 – 1989
|
| Keywords | Glucocorticoid / Glucocorticoid receptors / Cortisol resistance / Fibroblast, Epstein-Barr virus transforned B-ltymphoytes / 繊維芽細胞 / EーBウィルス |
|---|
| Research Abstract |
In 1985, we found a family (the mother and son) with cortisol resistance. Through investigations of literature revealed that the family was the second family in the world with this disorder. Thus, our study confirmed the presence of such a clinical entity in human beings. Plasma cortisol levels of these patients were surprisingly high in the absence of any of clinical manifestations of cushing's syndrome. The number of glucocorticoid receptors in peripheral blood mononuclear cells of the patients was decreased to 50% of normal subjects.
In order to demonstrate the resistance of the patient's cells to glucucurticoid, in vitro, we cultured the patient's skin fibroblasts and stdied the effect of dexamethasone on the proliferation of the cells. We successfully demonstrated that higher concentrations of dexamethasone were required to suppress thymidine uptake by the patient's cells than normal fibroblasts. The results indicate that the patient's cells are resistant to glucocorticoids.
To further elucidate the nature of glucocorticoid receptors in the patients, we established Epstein-Barr virus transtormed B-lymphocytes clones. In these cells, the number of glucocorticoid receptors was also decreased to one-half of normals. This finding provided evidence that the genetical abnormality was also expressed in these cells and the cells had potential advantages for further genetical analyses.
|
