1988 Fiscal Year Final Research Report Summary
Analysis of genes involved in proliferation and differentiation of megakaryopoiesis using a human megakaryoblastic leukemia cell line
| Project/Area Number |
62480261
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Nagoya University School of Medicine |
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Principal Investigator |
OHNO Ryuzo Nagoya University School of Medicine, 医学部, 講師 (70093002)
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| Co-Investigator(Kenkyū-buntansha) |
TANIMOTO Mitsune Nagoya University School of Medicine, 医学部, 医員
OGURA Michinori Nagoya University School of Medicine, 医学部, 医員 (40231229)
MORISHIMA Yasuo Nagoya University School of Medicine, 医学部, 助手 (20220056)
SAITO Hidehiko Nagoya University School of Medicine, 医学部, 教授 (20153819)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Megakaryoblastic cell line / Megakaryocyte / MEG-01 / Culture cell line / Oncogene / Differentiation induction / Phorbol esters / TPA / C-myc / C-fos / cーfos / cーsis |
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| Research Abstract |
Expression of c-abl, c-fos, c-sis and myc gene family (c-myc, L-myc and N-myc) was examined during 12-O-tetradecanoyl-phorbol-13-acetate (TPA) induced maturation in a newly established human acute megakaryoblastic leukemia cell line (MEG-01), which shows dominant morphological changes and/or productions of cell lineage specific functional proteins after TPA treatment. Upon maturation induction, constitutive expression of c-myc oncogene in the cell line was rapidly decreased and become fairly undetectable within 4 h. The level of c-myc was fully recovered after 24 h in MEG-01 cells, whereas it remained undetectable in NOMO-1 cells, a human acute monoblastic leukemia cell line. C-fos RNA was induced within 15 min after TPA treatment, reached to the maximum transcriptional level in 1 h and thereafter decreased. Expression of c-abl, L-myc and N-myc was detected in neither uninduced nor induced condition. Actinomycin D treatment on TPA-induced cells made no effect on the induced reduction of c-myc expression in MEG-01 cells, whereas the stability of c-myc transcripts in NOMO-1 cells was decreased by the presence of actinomycin D. DNAse I treatment of nucleic DNA of both uninduced and induced cell lines showed that the loss of a single DNAse I hypersensitive site 5' of the c-myc gene was accompanied with reduced c-myc expression in NOMO-1 cells.
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