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1989 Fiscal Year Final Research Report Summary

Fundamental Studies to Elucidate Cerebral Developmental Impairment Mechanisms in Congenital Hydrocephalus and Development of Intrauterine Treatment.

Research Project

Project/Area Number 62480312
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionJuntendo University

Principal Investigator

SATO Kiyoshi  Department of Neurosurgery, Professor, 医学部脳神経外科, 教授 (10112707)

Co-Investigator(Kenkyū-buntansha) SUDA Kikuo  Department of Neurosurgery, Assistant Researcher, 医学部脳神経外科, 助手 (00206559)
TAKEDA Nobuaki  Department of Neurosurgery, Assistant Researcher, 医学部脳神経外科, 助手 (00171645)
ITO Masanori  Department of Neurosurgery, Assistant Professor, 医学部脳神経外科, 講師 (20095764)
MIYAOKA Makoto  Department of Neurosurgery, Assistant Professor, 医学部脳神経外科, 講師 (70053270)
Project Period (FY) 1987 – 1989
Keywordscongenital hydrocephalus / congenital hydrocephalic rat / ventriculography / magnetic resonance imaging / ^<14>C-deoxyglucose / Golgi study / ventriculoperitoneal shunt / cerebral cortex
Research Abstract

We have experimentally treated the hydrocephalus of congenitally hydrocephalic HTX-rats by inserting a ventriculoperitoneal shunt (V-P shunt), at different times after birth, investigated through neuropathology and neurochemistry clarification of how timing of such surgery modifies impairment in development of neurocytes and synaptogenesis, and assessed the learning ability of the experimental animals.
Experimental results obtained in 1987 suggested that placement of the V-P shunt delayed until around 4 weeks after birth of the hydrocephalic animals did not correct impairment of synaptogenesis of cortical pyramidal neurons and learning disability in comparison with non-hydrocephalic HTX-rats. In light of such observations, earlier placement of the V-P shunt at around 7 days after birth was carried out in experiments conducted in 1988 and 1989. The significance of the early placement was evaluated by various means, i.e., (1) chronological changes in lateral ventricle size and thickness o … More f the cortical mantle of the brain by means of Isovist ventriculography and magnetic resonance imaging(MRI), (2) measurement by 14C-deoxyglucose autoradiography of local cerebral glucose utilization (LCGU), (3) neuropathological techniques including rapid Golgi staining, (4) immunohistochemical assessment of synaptogenesis using monoclonal antibodies against a synaptic vesicle protein (SVP-38 of Obata et al., 1986), and (5) observation of learning ability by means of the Y-Arm-Maze Test.
The results revealed that early placement of the V-P shunt was obviously effective in correcting reduced width of the cortical mantle and dilated ventricular size, mitigating cerebrospinal fluid white matter edema, normalizing the shape and arrangement of the ependymal cells lining the ventricular walls, preventing impairment of LCGU, restoring spine density of cerebral pyramidal neurons, and in decreasing learning disability. The present investigation would appear to have established a theoretical base supporting the necessity for early placement of a V-P shunt in animals with congenital hydrocephalus.
Furthermore, the results may urge reconsideration of intrauterine treatment of congenital hydrocephalus in humans which a majority of neurosurgeons already have abandoned. Less

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 宮澤隆仁他: "先天性水頭症ラット-HTX-における大脳皮質神経細胞の発達障害について-Golgi study-" 小児の脳神経. 13. 263-270 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 宮岡誠他: "先天性水頭症ラットに対するV-Pシャント前・後における脳局所グルコ-ス代謝" 小児の脳神経. 13. 389-393 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wada M.: "Congenital hydrocephalus in HTX-rats:incidence,pathophisiology,and developmental impairment." Neurologia medico-chirurgica. 28. 955-964 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyaoka M.etal: "Measurement of local cerebral glucose utilization befor and after V-P shunt in congenital hyrocephalusin rats." Metabolic Brain Disease. 3. 125-132 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyazawa T.et al: "A new method of quantitatively analyzing synapsis using a synaptic vesicle protein(SVP-38)monoclonalantibody." in preparation.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyazawa t.et al: "Impairment of synaptogenesis and learning disability in theHTX-rats with arrested sunt-dependent hydrocephalus." in preparation.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤潔他句: "水頭症-基礎と臨床-先天性水頭モデルの病態(出版予定)" 松本 悟(永井書店),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wada M: "Congenital hydrocephalus in HTX-rats: incidence, pathophysiology, and developmental impairment." Neurologia medico-chirurgica 28, 955-964, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyaoka M. et al: "Measurement of local cerebral glucose utilization before and after V-P shunt in congenital hydrocephalus in rats." Metabolic Brain Disease 3, 125-132, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyazawa T. et al: "A new method of quantitatively analyzing synapses using a synaptic vesicle protein (SVP-38) monoclonal antibody."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyazawa T. et al: "Impairment of synaptogenesis and learning disability in the HTX-rats with arrested shunt-dependent hydrocephalus."

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyazawa T. et al: "A quantitative Golgi study of cortical pyramidal neurons in congenitally hydrocephalic rats-HTX." Nervous System in Children 13, 263-270, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyaoka M. et al: "The effect of V-P shunt on local cerebral glucose utilization in HTX congenital hydrocephalic rats." Nervous System in Children 13, 389-393, 1988.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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