1989 Fiscal Year Final Research Report Summary
The Expression of Oncogenes in the Trophoblast and fetus of Normal Pregnancy
| Project/Area Number |
62480352
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | The Tokyo Jikeikai University School of Medicine |
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Principal Investigator |
YOKOYAMA Shiro Tokyo Jikeikai Univ.School of Medicine OB&Gy, Assist. Prof., 医学部(産婦人科), 助手 (20142490)
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| Project Period (FY) |
1987 – 1989
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| Keywords | C-myc oncogene / C-sis ancogene / C-fms oncogene / Trophoblast / Differentiation / Growth / Trophoblastic disease / 絨毛性 |
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| Research Abstract |
Recently it has been suggested that proto-oncogene plays a role not only in cellular proliferation, development and differentiation, but also in neoplastic transformation.
We now show the expression and its localization of c-myc, c-fms and c-sis proto-oncogenes in human developing chorionic tissue and fresh surgical specimens of mole and choriocarcinoma with the method of Northern blotting and In-situ hybridization. The 2.4kb c-myc transcript has been localized to the cytotrophoblast in early placenta and also localized to the C and S type trophoblastic cells in mole and choriocarcinoma. The 4.0kb c-fms transcript has been localized to the syncytiotrophoblast, especially the highly differentiated syncytiotrophoblast in the second and third trimesters and S typed trophoblastic cells in mole and choriocarcinoma. Moreover, the 4.0kb c-sis transcript has been localized to the cytotrophoblast in early placenta, but not detected in mole or choriocarcinoma. First, these results suggest that the stage and site specific expression of c-myc, c-fms and c-sis proto-oncogenes are clearly related to the proliferation, development and differentiation of normal trophoblastic cells. Second, the expression of c-myc, c-fma proto oncogenes may be of particular importance in the tumorigenesis and progression of trophoblastic disease.
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