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1988 Fiscal Year Final Research Report Summary

Roles of lysosomal proteinases in the patho-physiological changes of hard tissues.

Research Project

Project/Area Number 62480381
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionNagasaki University

Principal Investigator

KATO Yuzo  Nagasaki University School of Dentistry, 歯学部, 教授 (20014128)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Miho  Nagasaki University School of Dentistry, 歯学部, 助手 (50191341)
YAMAMOTO Kenji  Nagasaki University School of Dentistry, 歯学部, 助教授 (40091326)
Project Period (FY) 1987 – 1988
KeywordsLysosomal proteinases / Cathepsin B / Cathepsin H / Cathepsin D / Cathepsin E / カテプシンL / メダラシン
Research Abstract

Recent studies have suggested that a variety of lysosmal proteinases play an importantrrole in destructive lesion of hard tissues. However, a fundamental question regarding the mechanism of destructive process of hard tissue proteins still remains to be answered. The present studies was undertaken to prepare various lysosomal proteinases and antibodies specific for these enzymes for defining the properties of degradative system responsible for the breakdown of the tissue proteins. In the fiscal year of 1987, the work was concerned chiefly with lysosomal cysteine proteinases cathepsin B and cathepsin H. Preparation of the pure enzymes and their antibodies was accomplished in this year. Comparative analyses with various protease inhibitors and anti-inflammatory agents indicated the difference in catalytic site properties between cathepsins B and H. In the fiscal year of 1988, attention was focused on intracellular aspartic proteinases cathepsin D and cathepsin E. Immunochemical and immunohistochemical studies revealed that these two enzymes had the different distribution and subcellular localization in various tissues and blood cells. cathepsin d was detected in all of the tissues and cells tested, whereas cathepsin E had a relatively limited distribution. In addition, cathepsin D was associated with the lysosome-rich fraction, whereas cathepsin E behaved as a soluble cytosolic enzyme.
All through the two years, possible roles of lysosomal proteinases in pathological destructive process of periodontal tissues were investigated. The results obtained from analyses of the gingival crevicular fluid from chronic adult periodontitis patients who had rediographic evidence of alveolar bone loss indicated that the levels of cathepsins B, H, L and medullasin was correlated

  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Yamamoto,Kenji: Biochem.Biophys.Res.Commun.148. 267-272 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 国松和司: 日本歯周病学会会誌. 29. 1094-1101 (1987)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,Kenji: Biol.Chem.Hoppe-Seyler. 369. 315-322 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] 山本健二: 炎症. 8. 217-222 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] 山本健二: 日本薬理学会誌. 91. 371-378 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jupp.Ray: Biochem.J.254. 895-898 (1988)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,Kenji: J.Biochem.105. 114-119 (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] Thomas,J.: FEBS Lett.(1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 国松和司: 炎症. 9. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本健二: 炎症. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakai,Hideaki: Biochim.Biophys.Acta.105. (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] Ueno,Eiko: J.Biochem.105. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤有三: "鎮痛抗炎症剤の副作用" 国際医書出版(歯科ジャーナル), 755-760 (1987)

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      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto,Kenji: "Intracellular Protein Catabolism" Springer-Verlag & Japan.Sci.Soc.Press., (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, Kenji: "Biochemical and immunochemical similarity between erythrocyte membrane aspartic proteinase and cathepsin E." Biochem. Biophys. Res. Commun.148. 267-272 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunimatsu, Kazushi: "Studies on the roles of lysosomal proteinases in periodontal breakdown." J. Japan. Ass. Periodont.29. 1094-1101 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, Kenji: "An aspartic proteinase of erythrocyte membranes. Proposed mechanism for activation and further molecular properties." Biol. Chem. Hoppe-Seyler. 369. 315-322 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, Kenji: "Erythrocyte membrane aspartic proteinase(EMAP). Properties and biological controls." Jpn. J. Inflam.8. 217-222 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, Kenji: "Differential effects of non-steroidal anti-inflammatory drugs on cathepsins D and E from rat spleen." Folia pharmacol. japon.91. 371-376 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jupp, Ray: "Identification of the slow-moving proteinase from human gastric mucosa and the aspartic proteinase of erythrocyte membranes as catalytically equivalent to cathepsin E." Biochem. J.254. 895-898 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, Kenji: "Age-related and phenylhydrazine-induced activation of the membrane-associated cathepsin E in human erythrocytes." J. Biochem.105. 114-119 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Thomas, J.: "Stabilization of cathepsin E by ATP." FEBS Lett.(1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunimatsu, Kazushi: "Cathepsins B, H and L activities in gingival crevicular fluid from patients with chronic adult periodontitis." Jpn. J. Inflam.9. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, Kenji: "Gingival crevicular fluid level of medullasin, an elastase-like serine proteinase." Jpn. J. Inflam.9. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakai, Hideaki: "Quantitation and immunohistochemical localization of cathepsins E and D in rat tissues and blood cells." Biochim. Biophys. Acta. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueno, Eiko: "Activation mechanism for erythrocyte cathepsin E. Evidence for the occurrence of membrane-associated active enzyme." J. Biochem.105. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, Yuzo: "Hypocalcemic action of several types of salicylic acid analogues." Jpn. J. Oral Biol.31. 89-94 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, Yuzo: Side Effects of Analgesic Anti-Inflammatory Drugs.Kokusai Isho Shuppan, 755-760 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, Kenji: Intracellular Protein Catabolism.Springer-Verlag & Japan. Sci. Soc. Press, (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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