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1989 Fiscal Year Final Research Report Summary

Selection of liposomes used for drug delivery systems by double fluorescent labeling method

Research Project

Project/Area Number 62480422
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionKyushu University

Principal Investigator

GOTO Shigeru  Faculty of Pharmaceutical sciences, Kyushu University, Professor, 薬学部, 教授 (20037576)

Co-Investigator(Kenkyū-buntansha) KINOSHITA Nadao  School of Health Sciences, Kyushu University, Professor, 医療技術短期大学部, 教授 (10037351)
Project Period (FY) 1987 – 1989
Keywordsliposome / calcein / carboxyfluorescein / phosphatidylcholine / cholesterol / double fluorescent labeling method / particle size / body distribution
Research Abstract

A new method to evaluate the distribution of liposome expected to be a useful drug delivery system (DDS) has been devised. Two kinds of fluorescent substances which enable fractional determination by a simple process are chosen and separately enclosed in two kinds of liposomes having different characters, then they are given to an animal so that the distribution of two kinds of liposomes on the animal can be compared and examined simultaneously. This method also allows the accurate comparison of the distribution of two kinds of liposomes by minimum number of animal experiments not being puzzled by individual differences of animals.
Utilizing the characteristic of fluorescence of calcein (CAL) which is quenched with C0^<2+> ion, fractional determination conditions have been established for a combination of CAL with an unquenchable fluorescent substance, carboxyfluorescein (CF). In the experiment using mice, the result obtained showed the potential usefulness of this method for the study of blood concentration and body distribution of liposomes having different mean particle size in vivo. Following rapid i.v. of liposome that the mean particle size is 60 nm, the rate of loss of liposome from-the blood was most slowly.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 澤原英幸,後藤茂,木下洋夫: "Double fluorescent labeling method using for liposome stuey" Chemical and Pharmaceutical Bullentin(投稿中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideyuki Sawahara, Shigeru Goto, Nadao Kinoshita: "Double fluorescent labeling method using for liposome study" Chemical and Pharmaceutical Bulletin.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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