• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1988 Fiscal Year Final Research Report Summary

Structures and biological activities of novel phosphonoglycosphingolipids.

Research Project

Project/Area Number 62480450
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionNiigata University

Principal Investigator

SATAKE Mei  Brain Research Institute, Niigata University, Professor, 脳研究所, 教授 (70018589)

Co-Investigator(Kenkyū-buntansha) WATANABE Yoko  College of Biomedical Technology, Niigata University, Assistant, 医療技術短期大学部, 助手 (80018853)
ABE Sachiko  Brain Research Institute, Niigata University, Assistant, 脳研究所, 助手 (60018603)
ANDO Susumu  Tokyo Metropolitan Institute of Gerontology, Leader, 生化学部, 室長 (30073000)
ARAKI Shigeko  Brain Research Institute, Niigata University, Assistant, 脳研究所, 助手 (70018604)
Project Period (FY) 1987 – 1988
KeywordsPhosphonosphingoglycolipid / Pyruvate linked galactose / Nerve bundle in Aplysia / Chemical structure / あめふらし神経内局在
Research Abstract

In the present study, chemical structure and histohogical localization of a major phosphonoglycosphingolipid of nerve fibes of Aplysia kurodai, a sea gastropod, were identified.
1. Chemical structure of FGL-IIb: The complete structure was determined as 3,4-0-(1-carboxy-ethylidene)Ga1 1 3GalNAc 1 3[Fuc 1 2](2-aminoethylphosphony1 6)Ga1 1 4Glc 1 1-ceramide using GC-MS, NMR and chemical methods including permethylation etc. This is the first pyruvate containing sphingolipid so far reported in the animal. The basic structure of FGL-IIb is the same with that reported by us for the three major phosphonoglycosphingolipids of the skin of Aplysia, namely N-Ac galactosaminyl 1 3lactosy1-1 ceramide. Pyruvate in the molecule may make FGL-IIb more acidic than other phosphonoglycosphingolipids identified by us.
2. Tissue distribution of FGL-IIb and immunochemically related glycolipids: Anti FGL-IIb antiserum was raised in the rabbit and this polyclonal antiserum was used for immunochemical and immunohistochemical studies with HRP staining. Narve bundles in the nerve fibers, ganglia and skin were stained. Other components including nerve cell bodies and neuropil were not stained at all. The reactivity of FGL-IIb to the antiserum was abolished by esterification of FGL-IIb and the lost activity was recovered by saponifiction. This indicates that free carboxyl of the pyruvic acid of FGL-IIb is responsible for the antigenicity of FGL-IIb.
Besides FGL-IIb, other phosphonoglycosphingolipids identified in the nerve fiber of Aplysia as FGL-I, FGL-IIa, FGL-V and F-9 were also stained with the antiserum on TIC indicating that these glycolipids share the same epitope with FGL-IIb against the antibody. Pretreatment of tissues with chloroform-methanol mixture completely abolished the staining of nerve bundles.
These results reveal that only the nerve bundles in Aplysia tissues contain very acidic glycolipids as FGL-IIb, indicating some neurobiological significances of these glycolipids.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Shigeko Araki: J.Biochem.101. 145-152 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeko Araki: J.Biol.Chem. 262. 14141-14145 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sachiko Abe: J.Biochem.104. 220-226 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Heinz Beitinger: J.Biochem.105. 730-735 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeko Araki: "The Third Rinsho-ken International Conference Abstracts" Secretariat-Rinshoken Conference,The Tokyo Metropolitan Institute of Medical Science, 157 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeko Araki: "Isolation and characterization of a novel 2-aminoethylphosphonylglycosphingolipid from the sea hare, Aplysia kurodai." J. Biochem.101. 145-152 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeko Araki: "Structure of a triphosphonopentaosylceramide containing 4-O-methyl-N-acetylglucosamine from the skin of the sea hare, Aplysia kurodai." J. Biol. Chem.262. 14141-14145 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sachiko Abe: "Immunochemical and histochemical studies on a phosphonoglycosphingolipid, SGL-II, isolated from the sea gastropod Aplysia kurodai." J. Biochem.104. 220-226 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Heinz Beitinger: "Comparative monolayer investigations of surface properties of negatively charged glycosphingolipids from vertebrates (gangliosides) and invertebrates (SGL-II, lipid IV)." J. Biochem.105. 730-735 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeko Araki: The Third Rinsho-ken International Conference Abstracts. Secretariat-Rinshoken Conference, The Tokyo Metropolitan Institute of Medical Science, (1988)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1990-03-20  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi