1988 Fiscal Year Final Research Report Summary
Hemolymph Coagulation and Defence Systems in Invertebrate Animals
| Project/Area Number |
62480453
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Kyushu University |
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Principal Investigator |
IWANAGA Sadaaki Faculty of Science, Kyushu University, Professor, 理学部, 教授 (90029942)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Toshiyuki Faculty of Science, Kyushu University, Research Associate, 理学部, 助手 (90183970)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Hemolymph Coagulation / Horseshoe Crab Hemocyte / Lipopolysaccharide / Factor C / _2-GP-I domain / EGF domain / Tachyplesin / セリンプロテアーゼチモーゲン |
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| Research Abstract |
The horseshoe crab clotting factor, factor C, present in the hemocytes is a serine-protease zymogen activated with lipopolysaccharide. It is a two-chain glycoprotein (M_r = 123,000) composed of a heavy chain (M_r = 80,000) and a light chain (M_r = 43,000) [T. Nakamura et al. (1986) Eur. J. Biochem. 154, 511-521]. In our continued study of this zymogen, we have now also found a single-chain form of factor C (M_r = 123,000) in the hemocyte lysate. the heavy chain had the NH_2-terminal sequence of Ser-Gly-Val-Asp-, consistent with that of the single- chain factor C, indicating that the heavy chain is derived from the NH_2-terminal part of the molecule. The light chain had an NH_2-terminal sequence of Ser-Ser-Gln-Pro-. Incubation of the two-chain zymogen with lipopolysaccharide resulted in the cleavage of a Phe-Ile bond between residues 72 and 73 of the light chain. Concomitant with this cleavage, the A (72 amino acid residues) and B chains derived from the light chain were formed. The complete amino acid sequence of the A chain was determined by automated edman degradation. The A chain contained a typical segment which is similar in sequence to a family of repeats in human _2-glycoprotein I, complement factors B, protein H, C4b-binding protein, and coagulation factor XIII b subunit. The NH_2-terminal sequence of the B chain was Ile-Trp-Asn-Gly-. This chain contained the serine-active site sequence-Asp-Ala-Cys-Ser-Gly-Asp-Ser-Gly-Gly-Pro-.
These results indicate that horseshoe crab factor C exists in the hemocytes in a single-chain zymogen from and is converted to an acitve serine protease by hydrolysis of a specific Phe-Ile peptide bond.
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