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1988 Fiscal Year Final Research Report Summary

Metabolism in root nodules and function of leghemoglobin

Research Project

Project/Area Number 62490009
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionNagoya University

Principal Investigator

YAMAMOTO Yukio  Professor, School of Agriculture, Nagoya University, 農学部, 教授 (40022561)

Project Period (FY) 1987 – 1988
KeywordsNodule / Leghemoglobin / Soy bean / Symbiotic nitrogen fixation / Bacteroid / Uricase / Xanthine dehydrogenase / 根粒の代謝
Research Abstract

Soybean nodules consist of cells infected and uninfected with Bradyrhizobium japonicum. The infected cells are large and filled with bacteroids. Nitrogen fixation in nodules supposedly proceeds with the interaction between infected and unifected cells. However, the relation of nitrogen-carbon metabolism and mutual supply of nitrogen- and carbon- compounds between both cells are little studied.
Protoplasts were prepared from nodules in several stages of soybean development. Infected cells, uninfected cells and cortex cells could be separated for the measurements of several enzyme activities. Xanthine dehydrogenase activity was found in infected cells. Uricase activity was found in uninfected cells. Phosphoenolpyruvate carboxylase (CO_2 dark fixation) activity was high in uninfected cells. Malic dehydrogenase activity was found in both infected and uninfected cells, but higher in infected cells.
The enzyme distribution indicates that the malate produced in CO_2-dark fixation of uninfected cells will be supplied to the infected cells and the uric acid produced in infected cells will be transported to the uninfected cells, where uric acid is converted to allantoin.
Calcium and boron stimulated nitrogen fixation when they were in optimum concentrations. The in vitro experiments showed that leghemoglobin elevated the respiration of mitochondria and bacteroids even under low oxygen concentrations.
Nitrate supply inhibited nitrogen fixation within 24 hours. The inhibition proceeded in accordance with the production of nitrite from nitrate. Leghemoglobin was converted to nitrosyl-leghemoglobin in the presence of nitrite. The O_2-binding of leghemoglobin was competitively inhigited by nitrite produced from nitrate.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Norio,Suganuma;Makoto,Kitou;Yukio,Yamamoto: Plant and Cell Physiology. 28(1). 113-122 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norio Suganuma;Yukio Yamamoto: Soil Science and Plant Nutrition. 33(1). 79-91 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norio Suganuma;Yukio Yamamoto: Soil Science and Plant Nutrition. 33(1). 93-101 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 礒井俊行,山本幸男: 日本土壌肥料学雑誌. 58(4). 405-409 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori Kanayama;Yukio Yamamoto: Plant Physiology. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori Kanayama;Yukio Yamamoto: Plant Physiology. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norio,Suganuma; Makoto,Kitou; Yukio Yamamoto: "Carbon metabolism in relation to cellular organization of soybean root nodules and respiration of mitochondria aided by leghemoglobin." Plan and Cell Physiology. 28 (1). 113-122 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Norio,Suganuma; Yukio,Yamamoto: "Carbon metabolism related to nitrogen fixation in soybean root nodules." Soil Science and Plant Nutrition. 33 (1). 79-91 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Norio,Suganuma; Yukio Yamamoto: "Respiratory metabolism of mitochondria in soybean root nodules." Soil Science and Plant Nutrition. 33 (1). 93-101 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki,Isoi; Yukio,Yamamoto: "Effects of calcium concentrations on initial growth, nodulation and nitrogen fixation in soybean plant" Japanese Journal of Soil Science and Plant Nutrition. 58 (4). 405-409 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshinori,Kanayama; Yukio Yamamoto: "Inhibition of nitrogen fixation by nitrate: Inhibition of function of leghemoglobin by nitrite produced from nitrate." Plant Physiology in preparation.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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