1988 Fiscal Year Final Research Report Summary
X-ray microanalytical and cytochemical studies on the calcium accumulation machanism.
| Project/Area Number |
62540570
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物形態・分類学
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| Research Institution | Yamaguchi University |
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Principal Investigator |
YAMAOKA Ikuo Yamaguchi University, Professor, 理学部, 教授 (30034705)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Ryutaro Yamaguchi University, Assistant, 理学部, 助手 (40182109)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Calcium accumulation / Alkaline phosphatase / Amoeba / Fibrocartilage / Os penis / Testosterone / X-ray microanalysis / 器官培養 |
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| Research Abstract |
Study on the calcification in the cell and tissue of animal organism has an important problem relating to the occification in the bone and dental formation.
In our project in order to understand the calcium accumulation mechanism two model systems were examined: one was a special granule containing calcium in an amoeba (Cochliopodium sp.), the other was the calcification in the fibrocartilage of the os penis of the rat.
In the amoeba the special granules were situated near the nucleus and were able to control the appearance rate. When the amoeba transplanted in the agar containing EGTA the special granule disappeared at hours 12. When the amoeba returened to the normal condition the granules reappeared, but not appeared in the agar containing levamisol which was a specific inhibitor to alkaline phosphatase activity (ALPase).
In the embrionec fibrocartilage cultured in vitro ALPase activities which depend on testosterone were detected at 14 days after culture. The calcium deposition occured in the matrix at 21 days.
These results suggest that the calcium accumulation mechabism relates to exsist of the alkaline phosphatase activity.
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