1988 Fiscal Year Final Research Report Summary
Fluorescence Correlation Spectroscopy as a Tool of Diffusional Study in Semi-Dilute Polymer Solution
| Project/Area Number |
62550655
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
高分子物性
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIOKAWA Kohzoh Research Associate, Faculty of Engineering, Kyushu University, 工学部, 助手 (70089928)
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| Co-Investigator(Kenkyū-buntansha) |
OYAMA Tomo-o Emeritus Professor, Kyushu University, 名誉教授 (80037663)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Fluorescence Correlation Spectroscopy / Correlator / Semi-Dilute Solution / 拡散 |
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| Research Abstract |
The fluorescence correlation spectroscopy has been proposed for determining diffusion coefficients. The experiment consists essentially in measuring variation of the number of fluorescent molecules in a defined open volume of solution. fluctuation of the number of small molecules is too small to measure. But fluctuation of the number of the polymer molecules, which contain many fluorescent group, can be detected.
The sample was irradiated with fine laser beam. The fluorescence was conducted to a photomultiplier by means of a single mode quartz optical fiber. Long pass filters were used to cut off the scattering of exiting light. The output signal of the photomultiplier was amplified and discriminated through a discriminator. The signal was counted by two counters alternately, and stored in the memory of the microcomputer. Then the correlation function was calculated.
The operational conditions were examined using fluorescent polystyrene beads as samples. The major experimental difficulty was the long integration time due to the low S/N ratio and the long correlation time. For reducing the correlation time, it is essential to reduce the cross section of the measuring volume. The drift of laser beam intensities interfered with the base lines of correlation functions.
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