1988 Fiscal Year Final Research Report Summary
Microbial glycosylation of vitamins and its application
| Project/Area Number |
62560108
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
発酵・醸造
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| Research Institution | Okayama University |
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Principal Investigator |
SUZUKI Yukio Research Institute for Bioresources, Okayama University, 資源生物科学研究所, 教授 (80033108)
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| Project Period (FY) |
1987 – 1988
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| Keywords | -Glucosyl-L-ascorbic acid / Sporobolomyces singularis / -Galactosylpyridoxine / -Fructosylpyridoxine / -Galactosyl-5-bromouridine / L-Ascorbic acid glucoside / Pyridoxine galactoside / ピリドキシン配糖体 / ヌクレオシド配糖体 |
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| Research Abstract |
1. Isolation and identification of a new glycoside of vitamin C. A new dervative of vitamin C, 6-0-( -glucosyl)-L-ascorbic acid, was found to be formed in a high yield (50%) when maltose and L-ascorbic acid were incubated with Aspergillus niger -gluco-sidase.
2. Microbial mass production of vitamin B_6-glycosides. (i) Formation of -glycosylpyridoxines in growing culture of Sporobolomyces singularis. 5'-0( -galactosyl)-pyridoxine, 4'-0-( -galactosyl)-pyridoxine, and 4'-0-( -digalactosyl)-pyridoxine were isolated in crystalline form in nearly 90% yield from the cultured broth of Sporobolomyces singularis grown on medium containing lactose and pyridoxine. (ii) Formation of -fructosylpyridoxine in growing culture of Aspergillus niger. A new derivative of pyridoxine, 5'-o-( -fructosyl)-pyridoxine, was found to be formed in growing culture containing sucrose and pyridoxine by Aspergillus niger.
3. Microbial mass production of glycosylnucleosides. Sporobolomyces singularis yielded large amounts of the -galactosides of 5-bromouridine and other nucleosides in growing cultures on media with lactose and these nucleosides.
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