1988 Fiscal Year Final Research Report Summary
Studies on the widening symbiotic specificity by the cell fusion of legume rhizobium
| Project/Area Number |
62560271
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産学(含草地学)
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| Research Institution | Miyazaki University |
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Principal Investigator |
IKEDA Hajime Miyazaki University ・ Faculty of Agriculture ・ Professer, 農学部, 教授 (20038194)
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| Co-Investigator(Kenkyū-buntansha) |
OYAMADA Masayuki Miyazaki University ・ Faculty of Agriculture ・ Assistant, 農学部, 助手 (60112413)
FUJII Noboru Miyazaki University ・ Faculty of Agriculture ・ Assistant Professer, 農学部, 助教授 (40040836)
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| Project Period (FY) |
1987 – 1988
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| Keywords | rhizobium / alfalfa / common lespedeza / acid tolerance / spheroplast / 細胞融合 |
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| Research Abstract |
For the purpose of breeding more acid tolerant isolates of Rhizobium meliloti on alfalfa plant, cell fusion of R.meliloti with Rhizobium sp. (common lespedeza) was tried.
The following results were obtained:
1)When each or R.meliloti and R.sp.(common lespedeza) were cultured on YM agar media with various pHs ranging 4.0 to 8.0, rhizobium sp.proliferated beyond pH value of 4.0, while proliferation of R.meliloti was not observed until pH 5.0.
2)As a result of comparing the method of spheroplast preparation for R.meliloti and R.sp., Rhizobium meliloti was spheroplasted by treating for 2 hrs with 200 mg/l lysozyme solution. On the contrary, R.sp.required 400 mg/l lysozyme for 2 hrs to obtain spheroplasts.
3)After cell fusion of spheroplasts of R.meliloti into those of R.sp.with 40%(W/V) polyethylene glycol 6000, cell fused rhizobia were isolated by 200 units/ml penicillin. Rhizobia isolated by treating with penicillin once for 6 hr or 3 hr were stimulated on YM agar medium pH 4.0 and they formed root nodules along the root of alfalfa.
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