• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1989 Fiscal Year Final Research Report Summary

Pharmacological study on intracerebral GABA mechanism

Research Project

Project/Area Number 62570095
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionTokyo Medical University

Principal Investigator

SATO Katsuhiko  Tokyo Med. Coll., Med. fac., Ass. Prof., 医学部, 助教授 (00133372)

Project Period (FY) 1987 – 1989
KeywordsGABA neuron / GAD neuron / immunohistochemistry / indirect fluorescent antibody technique / high-performance liquid chromatography / fluorescent histochemistry
Research Abstract

1. GABA immunohistochemistry. (1) Study of intraspinal GABA neuron The GABA neuron in the rat spinalcord issued yellowishgreen FITC-specific fluorescence. Specific fluorescence showing the localization of CABA-positive hells and GABA-positive fibers was observed in the spinal anterior horn and posterior horn. To compare the distribution of intraspinal GABA with that of a synthetic enzyme, we prepared specimens by an indirect fluorescent antibody technique using a GAD antibody, and studied for the distribution state on the same level. As a result, we found that the GAD distribution agreed with GABA localization, but the fluorescent intensity and distribution density were less than those of the specific fluorescence of GABA. Accordingly, we believe that using a GABA antibody directly is more accurate in histochemical ; searches for GABA-positive cells and positive fibers. (2) Study of intracerebral GABA neuron. We have studied immunohistochemically the nigra-striatal GABA pathway in the … More rat, and compared it with GAD localization, and compared it with GAD localization. As a result, GABA-positive cells and positive fibers issued green fluorescence in the stratum. Study of GAD-positive cells and positive fibers on the same level revealed that their distribution almost agreed with GABA distribution. GABA- positive fibers issued green fluorescence diffusedly at substantia nigra zoma compacta. The distribution of GAD-positive cells on the same level agreed with GABA distribution.
2. Biochemical analysis of GABA content in brain tissues. After pretreatment, the rat brain tissue sample to which a reaction reagent had been added was injected into 10-25ul HPLC. The peak width may become too large if the injection amount exceeds 25ul, with about 20-25 ng at 254 nm 0.01 AUF. The brain tissue sample showed a value of 18 ng/25 ul by a standard curve, and the retention time was 3.40 minutes. The total amount was 400 ul, which meant 288 ng/400ul. Accordingly the content in the sample was 2.88 ug/ml. In analysis of intracerebral GABA by HPLC, in this experiment a reliable and possible value appears to be a concentration of about 2 ug/ml. However, a study is still being made on it. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 渋谷健: "S-adenosyl-L-methionine sylfate tosylate(FO-1516)の薬理作用(第1報)ー中枢神経系におよぼす影響ー" 東京医科大学雑誌. 46. 460-470 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤勝彦: "S-adenosyl-L-methionine sylfate tosylate(FO-1516)の薬理作用(第2報)ー実験的脳虚血動物におよぼす影響ー" 東京医科大学雑誌. 46. 471-478 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渋谷健: "Ketanserin tartrate(KJKー945)の中枢作用(第1篇)" 東京医科大学雑誌. 46. 871-880 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渋谷健: "Ketanserin tartrate(KJKー945)の中枢作用(第2篇)" 東京医科大学雑誌. 46. 881-890 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤勝彦: "Ketanserin tartrate(KJKー945)の代謝産物,M-1,M-10及びM-12の中枢作用" 東京医科大学雑誌. 46. 891-897 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshi SHIBUYA: "Pharmacokinetics,Metabolism and Streoselectiv Biotransformation of Oral Alminoprofen in Humans" 臨床医薬. 5. 1141-1160 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshi SHIBUYA: "Pharmacological Actions of S-adenosyl-L-methionine Sulfate Tosylate (FO-1561) (First Report) -Effects on the central nervous system-" Journal of Tokyo Medical College Vol. 46, 460-470, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuhiko SATO: "Pharmacological Actions of S-adenosyl-L-methionine Sulfate Tosylate (FO-1561) (Second Report) -Effects on the experimental cerebral ischemic animals-" Journal of Tokyo Medical College Vol. 46, 471-478, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshi SHIBUYA: "Pharmacological Study on CNS Actions of Ketanserin Tartrate (KJK-945) (Part 1)" Journal of Tokyo Medical College Vol. 46, 871-880, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshi SHIBUYA: "Pharmacological Study on CNS Actions of Ketanserin Tartrate (KJK-945) (Part 2)" Journal of Tokyo Medical College Vol. 46, 881-890, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuhiko SATO: "Pharmacological Study on CNS Activities of Ketanserin Tartrate (KJK-945) metabolites M-1, M-10 and M-12" Journal of Tokyo Medical College Vol. 46, 891-897, 1988.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeshi SHIBUYA: "Study on Metabolism and Pharmacokinetics of Oral Alminoprofen (EB-382) in Healthy Volunteers" Journal of Clinical Therapeutics & Medicines Vol. 5, 1141-1160, 1989.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-03-26  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi