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1989 Fiscal Year Final Research Report Summary

Molecular biological study on modulatory effects of psychotropic agents on biosynthesis and metabolism of neuropeptides

Research Project

Project/Area Number 62570140
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionTokyo Institute of Psychiatry

Principal Investigator

YOSHIKAWA Kazuaki  PRIT, Dept. of Molecular Biology, Chief, 分子生物学研究室, 副参事研究員 (30094452)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Akihiro  PRIT, Dept. of Molecular Biology, Researcher, 分子生物学研究室, 研究員
AIZAWA Takako  PRIT, Dept. of Molecular Biology, Researcher, 分子生物学研究室, 研究員
KAMETANI Fuyuki  PRIT, Dept. of Molecular Biology, Researcher, 分子生物学研究室, 研究員 (70186013)
Project Period (FY) 1987 – 1989
KeywordsCholecystokinin / Enkephalin / Gene expression / Protein kinase A / Protein kinase C / Glucocorticoids / Second messenger systems / Psychotropic agents
Research Abstract

Various kinds of psychotropic agents exert their modulatory effects on neuronal functions through intracellular second messenger systems (e.g., protein kinases A and C). On the other hand, neuropeptides (i.e., endogenous, modulators of neuronal functions) are synthesized through the gene expression of their precursors. In this research project, we have attempted to examine modulatory roles of the second messenger systems in the gene expression of neuropeptide precursors. Major findings obtained are as follows:
1) In the rat telencephalon, the preprocholecystokinin gene expression is increased at early stages of postnatal development. In addition, there is a distinct sex-related difference in the gene expression, presumably due to effect of gonadal steroid hormones.
2) The expression of preproenkephalin gene in rat primary cultured cells is controlled by the activators for protein kinase A and C systems.
3) Glucocorticoids (i.e., steroid hormones having neurotropic actions) potentiate,the preproenkephalin gene activation mediated by protein kinase A system, but not that mediated by protein kinase C system.
4) The preproenkephalin gene is highly expressed in the rat germ cells, and the expression is hardly controlled by the protein kinase systems. The messenger RNA expressed in the germ cells shows a unique structure.
Above findings indicate that the expression of preproenkephalin gene is controlled by the interactions between intracellular second messenger systems. Gene expression of other neuropeptides may be controlled by similar mechanisms, and further studies should thus be planned.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Kazuaki Yoshikawa,Takako Aizawa: "Enkephalin precursor gene expression in postmeiotic germ cells" Biochemical and Biophysical Research Communications. 151. 664-671 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Yoshikawa,Takako Aizawa: "Expression of the enkephalin precursor gene in rat Sertoli cells:Regulation by follicle-stimulating hormone" FEBS Letters. 237. 183-186 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Yoshikawa,Takako Aizawa: "Transient increases and individual variations in preprocholecystokinin gene expression in rat telencephalic regions during postnatal development" Molecular Brain Research. 4. 87-96 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Yoshikawa,Kei Maruyama,Takako Aizawa,Akihiro Yamamoto: "A new species of enkephalin precursor mRNA with a distinct 5'-untranslated region in haploid germ cells" FEBS Letters. 246. 193-196 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Yoshikawa,Takako Aizawa,Akemi Nozawa: "Phorbol ester regulates the abundance of enkephalin precursor mRNA but not of amyloid beta protein precursor mRNA in rat testicular peritubular cellls" Biochemical and Biophysical Research Communications. 161. 568-575 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuaki Yoshikawa, Takako Aizawa: "Enkephalin precursor gene expression in postmeiotic germ cells" Biochem Biophys Res Commun 151, 664-671 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuaki Yoshikawa, Takako Aizawa: "Expression of the enkephalin precursor gene in rat Sertoli cells: Regulation by follicle-stimulating hormone" FEBS Letters 237, 183-186 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuaki Yoshikawa, Takako Aizawa: "Transient increases and individual variations in preprocholecystokinin gene expression in rat telencephalic regions during postnatal development" Mol Brain Res 4, 87-96 (1988).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuaki Yoshikawa, Kei Maruyama, Takako Aizawa, Akihiro Yamamoto: "A new species of enkephalin precursor mRNA with a distinct 5'-untranslated region in haploid germ cells" FEBS Letters 246, 193-196 (1989).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuaki Yoshikawa, Takako Aizawa, Akemi Nozawa: "Phorbol ester regulates the abundance of enkephalin precursor mRNA but not of amyloid beta protein precursor mRNA in rat testicular peritubular cells" Biochem Biophys Res Commun 161, 568-575 (1989).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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