1988 Fiscal Year Final Research Report Summary
Study on the mechanisms of receptor dysfunction with special reference to Basedow's disease
| Project/Area Number |
62570145
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Yamanashi Medical College |
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Principal Investigator |
KAWAOI Akira Professor Yamanashi Medical College, 医学部, 教授 (30059224)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Takao Assistant Te Yamanashi Medical College, 医学部, 助手 (70162443)
TASAKA Kachio Associate Yamanashi Medical College, 医学部, 助教授 (40093265)
USHIYAMA Hisashi Assista Yamanashi Medical College, 医学部, 助手 (60168680)
KOBAYASHI Makio Associat Yamanashi Medical College, 医学部, 助教授 (80060086)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Hormone Receptor / Receptor Dysfunction / Thyroid Gland / Basedow病 |
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| Research Abstract |
1. The binding sites on the thyroid cell membrane of the autoantibodies from the patients of autommunoe thyroid diseases were immunocytochemically demonstrated, using the cultured cells of human, porcine and rat thyroid Glands. These autoantibodies included TSAb, TBII and TSBAb types. The pattern of localization of antigenic sites was variable, such as diffuse and homogeneous type, pathchy distribution type and focal cytoplasmic process type depending on the conditions and systems of cell culture.
2. Horesradish peroxidase lablelled, and colloidal gold labelled TSH were successfully paalied as the ligands to localize the receptor site of TSH on the cultured thyroid cells.
3. The effects of antireceptor autoantibodies, TSAB, TBII and TSBAb on the binding of the labelled TSH with its receptor were examined. The results showed that there was a positive correlation between their antibody activity and the binding inhibitory activity of autoantibodies, suggesting that the stimulatory activity or TSH binding inhibitory activity could be demonstrable morphologically.
4. The polarity and function of thyroid cell membrane which are closely related with the expression of TSH receptor were investigated by the lectin binding method and enzyme histochemistry of thyroid peroxidase (TPO) and gamma-glutamyl transpeptidase (GGT). It was clarified that RCA lectin was useful as a marker of luminal surface of thyroid cell membrane, and that tpo and GGT were inverse in the expression on the thyroid cell membrane, the former as a marker of functional differentiation and the latter as an indicator of tumorigenesis in DIPH treated rat thyroid glands.
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