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1988 Fiscal Year Final Research Report Summary

Tissue-specific expression of the rearranged c-myc in murine plasmacytomas

Research Project

Project/Area Number 62570152
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionHokkaido University

Principal Investigator

OIKAWA Tsuneyuki  Hokkaido University School of Medicine, 医学部, 助手 (80150241)

Project Period (FY) 1987 – 1988
KeywordsMouse plasmacytoma / C-myc / Cell fusion / Hybrid / Tissue-specificity / DNase I感受生
Research Abstract

In a mouse plasmacytoma, not only the rearranged c-myc but also the non-rearranged c-myc was deregulated to be unresponsive to serum stimulation. Expression of the rearranged c-myc in the plasmacytoma was repressed after cell fusion with normal fibroblasts accompanied with suppression of tumorigenic activity. This repression of the rearranged c-myc could be also found in hybrids between plasmacytoma and liver cells or T-cell lymphoma but not in hybrids between plasmacytoma and B-cells, indicating involvement of tissue specific factors in the repression. Run-on assay showed the repression was at a transcriptional level. The repression was parallel to loss of plasmacytoma-specific proteins and reduced DNase I sensitivity of the rearranged c-myc in the hybrids. These results suggest that trans-acting factors from non-B cells act directly or indirectly to the rearranged c-myc in plasmacytomas to change chromatin configulation around the gene from open state to closed one.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Oikawa,T.,et al.: International Journal of Cancer. 39. 604-610 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondoh,N.,et al.: Leukemia Research. 11. 1149-1156 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada,T.,et al.: International Journal of Cancer. 40. 778-783 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oikawa,T.,et al.: International Journal of Cander. 42. 435-440 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kondoh,N.,et al.: Experimental Cell Research. 180. (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oikawa,T.,et al.: IARC Scientific publications. 92. 32-42 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oikawa, T. et al.: "Suppression of the transformed phenotypes in intraspecific somatic cell hybrid clones between the c-myc activationg mouse plasmacytoma line and normal cells." International Journal of Cancer. 39. 604-610 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondoh, N. et al.: "Unresponsiveness of both non-rearranged and rearranged c-myc to serum stimulation in a mouse plasmacytoma S194." Leukemia Research. 11. 1149-1156 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, T. et al.: "Absence of the hybrid bcr-abl mRNA in Ph-positive B lymphoblastoid cell line established from a patient with chronic myelogenous leukemia." International Journal of Cancer. 40. 778-783 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oikawa, T. et al.: "c-myc expression and transformed phenotypes in hybred clones between mouse plasmacytoma S194 cells and normal spleen cells or fibroblasts." International Journal of Cancer. 42. 435-440 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kondoh, N. et al.: "Reduced Dnase I sensitivity of the rearranged c-myc gene in somatic hybrids between murine plasmacytome cells and fibroblasts." Experimental Cell Research. 180. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oikawa, T. et al.: Suppression of transformed phenotypes and c-myc expression in a mouse plasmacytoma line after cell fusion with normal fibroblasts.IARC Scientific publication ,92, 32-42 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20  

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