1988 Fiscal Year Final Research Report Summary
Tissue-specific expression of the rearranged c-myc in murine plasmacytomas
| Project/Area Number |
62570152
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Hokkaido University |
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Principal Investigator |
OIKAWA Tsuneyuki Hokkaido University School of Medicine, 医学部, 助手 (80150241)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Mouse plasmacytoma / C-myc / Cell fusion / Hybrid / Tissue-specificity / DNase I感受生 |
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| Research Abstract |
In a mouse plasmacytoma, not only the rearranged c-myc but also the non-rearranged c-myc was deregulated to be unresponsive to serum stimulation. Expression of the rearranged c-myc in the plasmacytoma was repressed after cell fusion with normal fibroblasts accompanied with suppression of tumorigenic activity. This repression of the rearranged c-myc could be also found in hybrids between plasmacytoma and liver cells or T-cell lymphoma but not in hybrids between plasmacytoma and B-cells, indicating involvement of tissue specific factors in the repression. Run-on assay showed the repression was at a transcriptional level. The repression was parallel to loss of plasmacytoma-specific proteins and reduced DNase I sensitivity of the rearranged c-myc in the hybrids. These results suggest that trans-acting factors from non-B cells act directly or indirectly to the rearranged c-myc in plasmacytomas to change chromatin configulation around the gene from open state to closed one.
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