1988 Fiscal Year Final Research Report Summary
Study on proliferation and Differentiation of pluripotent hemopoietic stem cells (CFU-S).
| Project/Area Number |
62570166
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Department of Pathology, Yokohama City University School of Medicine |
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Principal Investigator |
INOUE Tohru M.D. Dept. of Pathol. Yokohama City University School of Medicine, 医学部病理学教室, 助教授 (50100110)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Hideki M.D. Dept. of Pediatrics, Yokohama City University School of Medicine, 医学部小児科学教室, 助手 (50106316)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Hemopoietic stem cells / CFU-S / spleen colony forming cells / transgenic mouse |
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| Research Abstract |
Shree independent programs are consisted this study, and each progress during the term of current fiscal year of the grant is as follows: 1. Pluripotent hemopoietic stem cells (CFU-S) and aging. Changes in hemopoietic microenvironment during aging were specifically focussed in terms of the effect on spleen colony formation. We investigated both CFU-Ss that were harvested from Dexter's liquid culture and that from bone marrow of recipient mice been repopulated with an old or a young bone marrow. One of experiments that came to a conclusion showed the responsibility to IL-3, of the CFU-s from old mice was not leser than that of control young mice, when bone marow cells that were harvested from old or yond donor, were cultured with IL-3 for 2 days, and let the cells from the culture form spleen colony. For further details, refer an abstract of the annual meeting of Japanese Hematological Society in Kyoto, 1988, presented by Y. Hirabayashi et al. 2. Heterogeneity of subfractions of CFU-S in radiosensitivity. This program was confirmed again by a series of three set of experiments, and the summary was presented at the International Conference on Biological Effects of Large Dose of Ionizing and Non-ionizing Radiation. 1988 in Hongzhou, China. 3. CFU-S and lymphogenous progenitor cells. In order to study this program, transgenic mice have been developed by a collaboration with Dr. Shin-ichi Aizawa (RIKEN) in which all of the hematopoietic stem cells have a marker that has been introduced. This will be presented at the annual meeting of Japanese Society of Pathology, 1989.
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