1988 Fiscal Year Final Research Report Summary
Molecular Structure and Function of Lyt-2 and Lyt-3
| Project/Area Number |
62570218
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Juntendo University |
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Principal Investigator |
HIRAMITSU Nakauchi Associate Professor, Juntendo Univ., 医学部, 講師 (40175485)
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| Project Period (FY) |
1987 – 1988
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| Keywords | CD8 / MHC class 1 / Lyt-2 / Lyt-3 |
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| Research Abstract |
In order to clarify the function of CD8 molecules, genes encoding Lyt-2 (CD8 a-chain) and Lyt-3(CD8 b-chain) were isolated and characterized. Molecular analysis of these genes revealed that 1) both Lyt-2 and Lyt-3 have homology to immunoglobulin V-region, 2) in addition to the homology to IgV-like region, Lyt-3 has IgJ-like region as well, 3)Lyt-3 gene spans a proximately 14Kbp and has 6 exons and 5 introns, 4)there is one base difference in the second exon between Lyt-3.1 and Lyt-3.2 genes which results in amino acid substitution of arginine (Lyt-3.1) to serine (Lyt 3.2), and this difference is responsible for the alloti picdifference of Lyt-3, 4)human homologue of Lyt-3 exists and has structu reshomologous to IgV, and IgJ regions, 5) both on mouse and human, expression of CD8 b-chain is dependent of CD8 a-chain, 6)in human, NK cells do not express CD 8 b-chain whereas some NK cells express CD8 a-chain only.
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