1988 Fiscal Year Final Research Report Summary
Analysis of Thymic Microenvironment for T lymphocyte Development
| Project/Area Number |
62570225
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | The National Children's Hospital, The National Children's Medical research Center |
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Principal Investigator |
MIZUTANI Shuki Department of Virology, The National Children's Medical Research Center, 小児医療研究センター・感染症研究部ウイルス (60126175)
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| Co-Investigator(Kenkyū-buntansha) |
藤本 純一郎 国立小児病院, 小児医療研究センター・病理病態研究部病理研, 室長 (60175578)
FUJIMOTO Junichiro Department of Pathology, The National Children's Medical Research Center (60175578)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Lymphokine / CALLA Antigen / DQ52 / IgH gene / 胸腺上皮細胞 |
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| Research Abstract |
In order to study the molecular mechanisms for early lymphocyte development, we have carried out the biochemical analysis of Common ALL antigen, which is expressed on the early stage of differentiation of B /or T lymphocyte. The result of our study indicates that the minor difference of CALLA molecule among differnt tissues is mainly due to those of syalidization.
In order to clarify the correlation of the differentiation antigen expression and genome organization of Ig heavy chain gene, we have studied the molecular features in 78 ALL patients. As the results of this study, we found B precursor ALLs with germ line configuration of IgH gene, those with the rearrangement of Domega52 and those with the rearrangement of D 5' to Domega52. We propose a model for the classification of early B lymphocyte based upon these findings. Thymic epithelial cell lines were established by using gene transfer technique. SV40 ori- gene was introduced into fetal thymic epithelia. As the results 2 cell lines were established. They produced M-CSF and experessed LFA3 molecule which is a lignad for CD2.
Our study is expected to help disclose the mechanisms for lymphocyte development in human.
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