1988 Fiscal Year Final Research Report Summary
Immunological analysis on anticancer mechanism in tumor tissue of gastrointestinal cancer patients
| Project/Area Number |
62570306
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
MITACHI Yasushi The Research Institute for Tuberculosis and Cancer, Tohoku University, 抗酸菌病研究所臨床癌化学療法部門, 助手 (50181920)
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| Co-Investigator(Kenkyū-buntansha) |
WAKUI Akira The Research Institute for Tuberculosis and Cancer, Tohoku University, 抗酸菌病研究所臨床癌化学療法部門, 教授 (20006076)
KAMBE Mariko The Research Institute for Tuberculosis and Cancer, Tohoku University, 抗酸菌病研究所附属病院癌化学療法科, 助手 (00195190)
ASAMURA Mitsuo The Research Institute for Tuberculosis and Cancer, Tohoku University, 抗酸菌病研究所附属病院癌化学療法科, 講師 (00124545)
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| Project Period (FY) |
1987 – 1988
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| Keywords | Gastrointestinal cancer / Immunohitochemical analysis / Tumor infiltrating lymphocytes / Cytotoxic T cell / Natural killer cell / Interferon / Interleukin-2 / Biological respnse modifiers |
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| Research Abstract |
An immunohistochemical study was performed on tumor tissue of gastrointestinal cancer patients, by avidin-biotin peroxidase complex method using monoclonal antibodies. Remarkable infiltration of CD4^+ lymphocytes (helper/inducer T cells) and moderate infiltration of CD8^+/CD11^- lymphocytes (cytotoxic T cells) were seen in the tissue of gastric cancer. Remarkable of moderate infiltration of CD16^+/CD11^+ lymphocytes (natural killer cells) were also found in the cancer tissue. Such patterns of lymphocyte infiltration indicate that these effector cells attack the gastric carcinoma. Incease in helper/inducer T cells are probably required to induce cytotoxic T cells.
Advanced gastrointestinal cancer patients were evaluated for changes in the absolute counts of peripheral blood lymphocytes, monocytes and lymphocyte subsets during chemotherapy, immunotherapy or immunochemotherapy. In the patients treated with chemotherapeutic agents, whole lymphocytes, monocytes, T cells, B cells, natural killer cells and T cell subsets decreased in number. In contrast, the counts of lymphocytes, monocytes and lymphocyte subsets showed little decrease in chemoimmunotherapy with BRM (biological response modifiers), and the percentage of activated T cells, cytotoxic T cells and natural killer cells increased to higher levels in treatment with interferon or interleukin-2.
It is suggested that the effector cells, such as cytotoxic T cells, natural killer cells and monocytes, are activated and mobilized into the cancer tissue by abministration of BRM, and demonstrate their antitumor activities.
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