1988 Fiscal Year Final Research Report Summary

Chromosomal analysis of woodchuck hepatocellular carcinoma utilizing in situ hybridization technique

Research Project

Project/Area Number	62570316
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Gastroenterology
Research Institution	Kanazawa University
Principal Investigator	KOBAYASHI Kenichi 1st. Dept. of Internal Medicine, School of Medicine,, 医学部, 助教授 (70019933)
Project Period (FY)	1987 – 1988
Keywords	Woodchuck hepatitis virus (WHV) / Hepatocellular carcinoma / Chromosomal abnormalities / In situ hybridization / WHVDNA組み込み部位
Research Abstract	The chromosomal sites of WHV DNA integration were identified in WH257 GE10 by an in situ hybridization technique. ^3H-labelled WHC DNA was assigned to the G-banded diagram which was determined for the first time in the woodchuck chromosomes. WHV DNA was integrated into two sites; 33 region of the long arm of chromosome 6 (6q33) and 31 region of the long arm of chromosome 8 (8q31). Chromosomal sites of WHV DNA integration were stable during the passage periods analyzed. These findings suggest that WHV DNA integration into the chromosomes of WH257 GE10 might be closely associated with the pathogenesis of a woodchuck hepatocellular carcinoma, and that integrated WHV DNA might play some role in the maintenance of the characteristics as cancer cells.