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1988 Fiscal Year Final Research Report Summary

Analysis of inhibitory mechanism by ethanol on liver regeneration.

Research Project

Project/Area Number 62570323
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionOsaka University

Principal Investigator

FUSAMOTO Hideyuki  Osaka University Medical School, Assistant, 医学部, 助手 (90124776)

Co-Investigator(Kenkyū-buntansha) 古澤 俊一  大阪大学, 医学部附属病院, 医員
SASAKI Yutaka  Osaka University Hospital, Resident, 医学部附属病院, 医員 (70235282)
KASAHARA Akinori  Osaka University Hospital, Resident, 医学部附属病院, 医員 (70214286)
FURUSAWA Shunichi  Osaka University Hospital, Resident
Project Period (FY) 1987 – 1988
Keywordsliver regeneration / proto-oncogene / 肝再生
Research Abstract

Expression of the proto-oncogenes such as c-fos and c-myc might play a role in the control of liver regeneration. In order to clarify the inhibitory mechanism of ethanol on hepatocytes proliferation, the expression of c-fos and c-myc and DNA synthesis were investigated. Rats were divided into 4 groups in this study. In the group A and B, rats were given water. In the group C and D, animals received 20% of ethanol ad libitum for 8 weeks. In group B and D, animals were given 33% of ethanol as a single dose of 4g ethanol /kg body weight to study a acute effect of ethanol. DNA synthesis as estimated by ^3H-thymidine incorporation was significantly inhibited only in chronically ethanol-fed rats with additional ethanol administration soon after partial hepatectomy, group D, whereas it was not changed in the other 3 groups. A significant reduction of c-myc transcripts was also found in chronically ethanol-fed rats with acute ethanol administration, group D, while c-fos expression was almost the same in the all groups.
It is concluded that the inhibition of liver regeneration by ethanol was observed in chronically ethanol-fed rats with acute ethanol administration, followed by a significant decrease in c-myc expression. This suggested that the reduction of c-myc expression was related to the inhibition of dna synthesis by ethanol.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 林紀夫、古沢俊一、鎌田武信: 生物物理化学. 31. 389-392 (1987)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 古沢俊一、林紀夫、八島俊、笠原彰紀、et al: 肝臓. 29. 377-381 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 八島俊、林紀夫、古沢俊一、笠原彰紀、et al: "アルコール代謝と肝 Vol.7 癌遺伝子発現動態からみたアルコールによる肝再生抑制メカニズムの検討" (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Yashima,;N.Hayasi,;A.Kasahara,et al: "Biomedical and Social Aspects of Alcohol and Alcoholism Inhibition of liver regeneration through decreased proto-oncogene expression by ethanol." Excerpta Medica, 737-740 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shunichi Furusawa; Norio Hayashi; Takashi Yashima; Akinori Kasahara; Yutaka Sasaki; Michikazu Kono; Kazuhiro Katayama; Mituyoshi Yamazoe; Hideyuki Fusamoto; Nobuhiro Sato; Takenobu Kamada: "Expression of proto-oncogenes during liver regeneration." Acta Hepatologica Japonica. 29. 377-381 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Yashima; N. Hayashi; A. Kasahara; S. Furusawa; Y. Sasaki; H. Meren; H. Fusamoto; N. Sato; T. Kamada: Inhibition of liver regeneration through decreased proto-oncogene expression by ethanol.Excerpta Medica, 737-740 (1988)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1990-03-20   Modified: 2017-04-05  

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