| Co-Investigator(Kenkyū-buntansha) |
BANDO Hisanori Mie University School of Medicine , Assistant, 医学部, 助手 (20189731)
TSURUDOME Masato Mie University School of Medicine , Assistant, 医学部, 助手 (50159042)
ITO Yasuhiko Mie University School of Medicine , Professor, 医学部, 教授 (00022872)
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| Research Abstract |
Human parainfluenza virus (PIV) caused respiratory tract infection of man in early life. Despite of their importance as human pathogen. Little is known of the structure and replication of the virus. PIVs are divided into 4 serological types, and type 4 viruses are subdivided into 4A (PIV-4A) and 4B (PIV-4B). PIV-4 has not been analyzed because of their poor growth characteristics in tissue culture. In the present study, we have established an experimental animal model for PIV-4 infection by using monkeys, produced monoclonal antibodies (MAbs), identified their structural proteins and analyzed the immunological cross-reactins among paramyxoviruses by using the MAbs.
We detected IgG and Ig, which are specific for PIV-4A or 4B in the infected mokey sera. The peak appearance of IgG was 2 weeks post re-infection (12 weeks post infection) and that of IgM was 2 weeks post infection. The apparance curves of neutralizing antibody and HI antibody were consistent with that of IgG. IgA and IgE were detected in the nasal washings and saliva, but not in sera, of the infected mokeys. The mol.wts. of the structural proteins of PIV-4A were as follows, HN:72K, NP:61K, F_0:61K, F_153K and 47K, and M:40K. Those of PIV-4b were, HN:72K, NP:65K, F_065K, F_155K and M:40K, HN and F proteins were glycoproteins. The cross-reactions among paramyxoviruses were determined. we found significant reactions between PIV-4A and 4B in BP protein, but relatively low reactions in HN, F and M proteins, and a few clones from PIV-4A and 4B have cross-reactions with PIV-2, mumps virus and simian virus 5. However, no cross-reactions were found among PIV-1, PIV-3, measles virus and NDV by using the MAbs to PIV-4A and 4B.
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